Electroacupuncture at Fengchi(GB20) and Yanglingquan(GB34) Ameliorates Paralgesia through Microglia-Mediated Neuroinflammation in a Rat Model of Migraine

作者全名："Zhou, Min; Pang, Fang; Liao, Dongmei; He, Xinlu; Yang, Yunhao; Tang, Chenglin"

作者地址："[Zhou, Min; Pang, Fang; Liao, Dongmei; He, Xinlu; Yang, Yunhao; Tang, Chenglin] Chongqing Med Univ, Coll Tradit Chinese Med, Chongqing 400016, Peoples R China; [Zhou, Min; Pang, Fang; Liao, Dongmei; He, Xinlu; Yang, Yunhao; Tang, Chenglin] Chongqing Key Lab Tradit Chinese Med Prevent & Cur, Chongqing 400016, Peoples R China"

通信作者："Tang, CL (通讯作者)，Chongqing Med Univ, Coll Tradit Chinese Med, Chongqing 400016, Peoples R China.; Tang, CL (通讯作者)，Chongqing Key Lab Tradit Chinese Med Prevent & Cur, Chongqing 400016, Peoples R China."

来源：BRAIN SCIENCES

ESI学科分类：NEUROSCIENCE & BEHAVIOR

WOS号：WOS:000979093900001

JCR分区：Q3

影响因子：2.7

年份：2023

卷号：13

期号：4

开始页：　

结束页：　

文献类型：Article

关键词：migraine; electroacupuncture; paralgesia; microglia; inflammatory response

摘要："Background: Multiple studies have suggested that paralgesia (hyperalgesia and cutaneous allodynia) in migraine reflects the activation and sensitisation of the trigeminovascular system (TGVS). In particular, it reflects the second-order and higher nerve centre sensitisation, which is caused and maintained by neuroinflammation. Microglia activation leads to the release of proinflammatory cytokines involved in inflammatory responses. Accumulating evidence indicates that electroacupuncture (EA) is effective in ameliorating paralgesia, but the underlying mechanisms of EA in migraine attacks caused by microglia and microglia-mediated inflammatory responses are still unclear. The purpose of this study was to explore whether EA could ameliorate the dysregulation of pain sensation by suppressing microglial activation and the resulting neuroinflammatory response, and to evaluate whether this response was regulated by Toll-like receptor 4 (TLR4)/nuclear factor-kappa B(NF-kappa B) in the trigeminal nucleus caudalis (TNC) in a rat model of migraine. Methods: Repeated Inflammatory Soup (IS) was infused into the dura for seven sessions to establish a recurrent migraine-like rat model, and EA treatment was administered at Fengchi (GB20) and Yanglingquan (GB34) after daily IS infusion. Facial mechanical withdrawal thresholds were measured to evaluate the change in pain perception, and plasma samples and the TNC tissues of rats were collected to examine the changes in calcitonin gene-related peptide (CGRP), the Ibal-1-labelled microglial activation, and the resulting inflammatory response, including interleukin-1 beta (IL-1 beta), tumour necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and their regulatory molecules TLR4/NF-kappa B, via enzyme-linked immunosorbent assay (ELISA), real-time polymerase chain reaction (RT-PCR), immunohistochemistry (IHC) and Western blot analysis. Results: Repeated IS injections into the dura induced facial mechanical paralgesia, which is the manifestation of migraine attacks, and increased the expression of CGRP, Ibal-1, microglial mediated inflammatory cytokines (IL-1 beta, TNF-alpha, IL-6), and regulatory molecules TLR4/NF-kappa B. EA at GB20/34 significantly attenuated repetitive IS-induced pain hypersensitivity. This effect was consistent with decreased levels of CGRP and inflammatory cytokines in the plasma and the TNC via the inhibition of microglia activation, and this response may be regulated by TLR4/NF-kappa B. Conclusions: EA ameliorated paralgesia in repetitive IS-induced migraine-like rats, which was mainly mediated by a reduction in microglial activation and microglial-mediated inflammatory responses that could be regulated by TLR4/NF-kappa B."

基金机构：Key Discipline Construction Project of Acupuncture Moxibustion and Massage of Chongqing Health Commission [472020320220021]; College of Traditional Chinese Medicine of Chongqing Medical University [2021-ZDXK-D04]; Joint Project of Chongqing Health and Family Planning Commission and Science and Technology Commission [ZY201801007]; Chongqing Yuzhong District Science and Technology Plan Project [20200116]

基金资助正文："This study was supported by the Key Discipline Construction Project of Acupuncture Moxibustion and Massage of Chongqing Health Commission (472020320220021), College of Traditional Chinese Medicine of Chongqing Medical University (No: 2021-ZDXK-D04), Joint Project of Chongqing Health and Family Planning Commission and Science and Technology Commission (No: ZY201801007), and Chongqing Yuzhong District Science and Technology Plan Project (No: 20200116)."