"Hsa_circ_0057104, by competitive adsorption of miR-627-5p, mediates CCND2 expression to promote malignant proliferation and Warburg effect of colorectal cancer"

作者全名："Zhang, Lin; Xu, Jian; Jiang, Dequan; Zhang, Jing; Li, Hongyuan; Zhao, Zhengzhong; Mei, Zhechuan"

作者地址："[Zhang, Lin; Jiang, Dequan; Zhang, Jing; Li, Hongyuan; Zhao, Zhengzhong; Mei, Zhechuan] Chongqing Med Univ, Dept Gastroenterol, Coll Clin Med 2, Chongqing, Peoples R China; [Xu, Jian] Chongqing Jiangjin Cent Hosp, Dept Gastroenterol, Chongqing, Peoples R China"

通信作者："Mei, ZC (通讯作者)，Chongqing Med Univ, Dept Gastroenterol, Coll Clin Med 2, Chongqing, Peoples R China."

来源：BIOTECHNOLOGY AND GENETIC ENGINEERING REVIEWS

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000979217200001

JCR分区：Q1

影响因子：6.5

年份：2023

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期号：　

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文献类型：Article; Early Access

关键词：CircPDK1; MiR-627-5p; CCND2; colorectal cancer; ceRNA

摘要："Objective: hsa_circ_0057104 (circPDK1) has been elucidated to regulate malignant behavior in pancreatic and renal cell carcinoma. The study functionally aimed at how circPDK1 modifies colorectal cancer (CRC) progression, along with its potential molecular mechanism.Methods: circPDK1 expression patterns in CRC tissues and cell lines were analyzed by RT-qPCR. circPDK1/miR-627-5p/CCND2 expression levels were changed by transient transfection. CCK-8 assay, flow cytometry, Transwell, immunoblotting, and commercial kits were utilized to measure CRC cell proliferation, apoptosis, invasion/migration, and glycolysis processes. Dual luciferase reporting assay and RIP assay were employed to evaluate the targeting relationship between circPDK1/miR-627-5p/CCND2.Results: circPDK1 was highly expressed in CRC. circPDK1 knockdown inhibited CRC cell proliferation, invasion/migration, and warburg effect and forced apoptosis. Overexpressing circPDK1 had the opposite effect. The effects of circPDK1 knockdown or circPDK1 overexpression on CRC cells were mitigated by downregulating miR-627-5p or CCND2, respectively. CircPDK1, by competitive adsorption of miR-627-5p, mediated CCND2 expression.Conclusion: CircPDK1 induces the malignant behavior of CRC by competitive adsorption of miR-627-5p mediating CCND2 expression, offering new insights into the future development of CRC-targeted drugs."

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