Electrochemiluminescence Aptasensor for HER2-ECD Detection Based on Resonance Energy Transfer between AuPt@ZIF-67 and AuNPs/g-C3N4/PDDA Composites

作者全名："Zhong, Hui; Yuan, Yonghua; Qing, Min; Zuo, Jianli; Li, Yueyuan; Bai, Lijuan"

作者地址："[Zhong, Hui; Zuo, Jianli; Li, Yueyuan; Bai, Lijuan] Chongqing Med Univ, Coll Pharm, Chongqing Res Ctr Pharmaceut Engn, Chongqing 400016, Peoples R China; [Yuan, Yonghua; Qing, Min] Chongqing Med Univ, Coll Pharm, Res Ctr Pharmacodynam Evaluat Engn Technol Chongqi, Chongqing 400016, Peoples R China"

通信作者："Bai, LJ (通讯作者)，Chongqing Med Univ, Coll Pharm, Chongqing Res Ctr Pharmaceut Engn, Chongqing 400016, Peoples R China."

来源：ACS APPLIED NANO MATERIALS

ESI学科分类：　

WOS号：WOS:000979662900001

JCR分区：Q2

影响因子：5.3

年份：2023

卷号：6

期号：8

开始页：6992

结束页：6999

文献类型：Article

关键词：breast cancer; HER2-ECD; electrochemiluminescence; resonance energy transfer; aptasensor

摘要："The extracellular domain of the human epidermal growth factor receptor (HER2-ECD) is a breast cancer biomarker. Accurate and sensitive detection of HER2-ECD concentration in serum is crucial in the diagnosis and patient follow-up. Herein, an ""off-on"" type electrochemiluminescence (ECL) aptasensor based on the resonance energy transfer (RET) between AuPt@ZIF-67 and AuNPs/g-C3N4/PDDA was developed for detecting HER2-ECD. First, AuNP-modified g-C3N4 (AuNPs/g-C3N4/PDDA), as a signal substrate, generates ECL signals under the co-reactant peroxodisulfate (S2O82-) in a specific potential range and shows significantly enhanced signal strength and stability. Then, AuPt@ ZIF-67 expeditiously quenched the ECL signal of AuNPs/g-C3N4/ PDDA because of ECL resonance energy transfer (ECL-RET) between AuPt@ZIF-67 and AuNPs/g-C3N4/PDDA. We designed a new aptasensor detection platform for HER2-ECD detection. After specific binding of the aptamer with HER2-ECD, the AuPt@ZIF-67 content in the system decreased, and the ECL response recovered. The biosensor demonstrated a linear range of HER2-ECD detection between 100 fg/mL and 100 ng/mL under ideal circumstances, with a low detection limit of 17.3 fg/mL. Furthermore, this biosensor was successfully tested in human serum samples. This strategy, as a novel ECL system with satisfactory stability and excellent sensitivity, can realize the detection of other biological molecules by changing the probe, which is a promising method."

基金机构："National Natural Science Foundation of China [82072378, 81601856]; Chongqing Talents: Exceptional Young Talents Project, China [CQYC202005015, cstc2021ycjh-bgzxm0328]; Ba Yu Scholar Program, China [YS2019020]; Program for Youth Innovation in Future Medicine, Chongqing Medical University, China [W0067]; Funds for High Level Young Science and Technology Talent Cultivation Plan in Chongqing Medical University, China; Discipline Talent Training Program of College of Pharmacy in Chongqing Medical University, China [YXY2019XSGG4]; Funds for Young Science and Technology Talent Cultivation Plan of Chongqing City [cstc2014kjrc-qnrc00004]"

基金资助正文："This work was supported by the National Natural Science Foundation of China (82072378, 81601856) ; Chongqing Talents: Exceptional Young Talents Project, China (CQYC202005015, cstc2021ycjh-bgzxm0328) ; Ba Yu Scholar Program, China (YS2019020) ; Program for Youth Innovation in Future Medicine, Chongqing Medical University, China (W0067) ; Funds for High Level Young Science and Technology Talent Cultivation Plan in Chongqing Medical University, China (2019) ; Discipline Talent Training Program of College of Pharmacy in Chongqing Medical University, China (YXY2019XSGG4) ; and Funds for Young Science and Technology Talent Cultivation Plan of Chongqing City (cstc2014kjrc-qnrc00004) ."