NUF2 is correlated with a poor prognosis and immune infiltration in clear cell renal cell carcinoma

作者全名："Zheng, Baishu; Wang, Shunde; Yuan, Xiaoyu; Zhang, Junyong; Shen, Zhongjie; Ge, Chengguo"

作者地址："[Zheng, Baishu; Yuan, Xiaoyu; Zhang, Junyong; Shen, Zhongjie; Ge, Chengguo] Chongqing Med Univ, Affiliated Hosp 2, Dept Urol, 76 Linjiang Rd, Chongqing 400010, Peoples R China; [Wang, Shunde] Chenjiaqiao Hosp Shapingba Dist, Dept Urol, Chongqing, Peoples R China"

通信作者："Ge, CG (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Dept Urol, 76 Linjiang Rd, Chongqing 400010, Peoples R China."

来源：BMC UROLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000979903000001

JCR分区：Q3

影响因子：1.7

年份：2023

卷号：23

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：NUF2; Clear cell renal cell carcinoma; Tumor immune infiltration; Immune cells

摘要："BackgroundClear cell renal cell carcinoma (ccRCC) is one of the most common malignancies. Recently, immunotherapy has been considered a promising treatment for metastatic ccRCC. NUF2 is a crucial component of the Ndc80 complex. NUF2 can stabilize microtubule attachment and is closely related to cell apoptosis and proliferation. This research is dedicated to investigating the role of NUF2 in ccRCC and the possible mechanisms.MethodsFirst, analysis of NUF2 mRNA expression levels in ccRCC and normal tissues by The Cancer Genome Atlas (TCGA) database and further verified by analysis of independent multiple microarray data sets in the Gene Expression Omnibus (GEO) database. Moreover, we evaluated and identified correlations between NUF2 expression, clinicopathologic variable, and overall survival (OS) in ccRCC by various methods. We investigated the relationship between NUF2 and tumor immune infiltration and the expression of corresponding immune cell markers via the Gene Expression Profiling Interactive Analysis (GEPIA) and Tumor Immune Estimation Resource (TIMER) databases. Then, we performed functional enrichment analysis of NUF2 co-expressed genes using R software and protein-protein interactions (PPIs) using the search tool used to retrieve interacting genes/proteins (STRING) databases.ResultsWe discovered that NUF2 mRNA expression was upregulated in ccRCC tissues and was associated with sex, grade, pathological stage, lymph node metastasis, and worse prognosis. In addition, NUF2 was positively linked to tumor immune cells in ccRCC. Moreover, NUF2 was closely related to genetic markers of different immune cells. Finally, functional enrichment and protein-protein interaction (PPI) analysis suggested that NUF2 and its closely related genes may be involved in the regulation of the cell cycle and mitosis. Our results suggested that NUF2 is correlated with a poor prognosis and immune infiltration in ccRCC."

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