Protective effect of additional cathelicidin antimicrobial peptide PR-39 on prosthetic-joint infections

作者全名："Zhu, Yongjun; Weng, Xuan; Zhang, Jian; Mao, Jingxin"

作者地址："[Zhu, Yongjun] Ninth Peoples Hosp Chongqing, Dept Orthoped, Chongqing, Peoples R China; [Zhu, Yongjun; Mao, Jingxin] Chongqing Med & Pharmaceut Coll, Dept Pharmacol, Chongqing, Peoples R China; [Weng, Xuan] Yubei Dist peoples Hosp Chongqing, Dept Orthoped, Chongqing, Peoples R China; [Zhang, Jian] Chongqing Med Univ, Dept Orthoped, First Affiliated Hosp, Chongqing, Peoples R China; [Mao, Jingxin] Southwest Univ, Coll Pharmaceut Sci, Chongqing, Peoples R China; [Zhang, Jian] Chongqing Med Univ, Dept Orthoped, First Affiliated Hosp, 1,Youyi Rd, Chongqing 400016, Peoples R China; [Mao, Jingxin] Chongqing Med & Pharmaceut Coll, Chongqing 400030, Peoples R China; [Mao, Jingxin] Southwest Univ, Coll Pharmaceut Sci, Chongqing 400715, Peoples R China"

通信作者："Zhang, J (通讯作者)，Chongqing Med Univ, Dept Orthoped, First Affiliated Hosp, 1,Youyi Rd, Chongqing 400016, Peoples R China.; Mao, JX (通讯作者)，Chongqing Med & Pharmaceut Coll, Chongqing 400030, Peoples R China.; Mao, JX (通讯作者)，Southwest Univ, Coll Pharmaceut Sci, Chongqing 400715, Peoples R China."

来源：JOURNAL OF ORTHOPAEDIC SURGERY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000981358600001

JCR分区：Q3

影响因子：1.3

年份：2023

卷号：31

期号：2

开始页：　

结束页：　

文献类型：Article

关键词：PR-39; antimicrobial peptide; infection; prosthetic joint; biofilm; bone marrow stem cells

摘要："BackgroundProsthetic-joint infection (PJI) is one of the severest complications after arthroplasty. However, antibiotics are not effective in the bacteria in biofilm outside the prosthetic-joint. Antimicrobial peptides have an efficient antimicrobial activity in staphylococcus aureus compared with conventional antibiotics.MethodsBone marrow stem cells (BMSCs) were isolated, cultured and transfected with cathelicidins antimicrobial peptides proline-arginine-rich 39 amino acid peptide (PR-39) lentivirus. The expression of PR-39 gene in BMSCs was detected by RT-PCR, and the antibacterial activity of PR-39 was measured by agar diffusion method. The transfection efficiency was detected by fluorescence microscopy. The infection model of artificial knee joint in rabbits were established. Kirschner wire was used as the knee joint implant to implant the distal femur through the femoral intercondylar fossa of rabbits. 24 rabbits were randomly divided into 2 groups for the above operations: group A was inoculated 0.5 mL into the joint cavity immediately after the incision was sutured 1 x 10(7) Staphylococcus aureus of colony forming unit (CFU), group B was inoculated with Staphylococcus aureus and PR-39. After operation, the wound conditions and histological changes were observed by X-ray and optical microscope respectively, CRP and erythrocyte sedimentation rate were measured by test assay.ResultsThe transfection efficiency of lentivirus vectortransfected BMSCs was 74.09%. The supernatant of lentivirus vector had obvious inhibitory effect on Staphylococcus aureus, and the antibacterial rate was 98.43%. 100% infection observed in group A while few infection observed in group B; serum CRP and ESR at a high level in group A while decreased in group B after operation. There were no significant difference in CRP and ESR between the pLV/PR-39 group and pLV/EGFP group at day 1 and 3 respectively after surgery. However, CRP and ESR in the pLV/PR-39 groupwere significantly lower than the pLV/EGFP group at day 7 and 14 respectively after operation.ConclusionsRabbits planted BMSCs expressing PR-39 were significantly increased resistance to Staphylococcus aureus in PJI than control group thus showing great potential for preventing implant-associated infection. It will provide a potential new therapeutic agent for implant-associated infection."

基金机构：　

基金资助正文：