Gossypin exert lipopolysaccharide induced lung inflammation via alteration of Nrf2/HO-1 and NF-kappa B signaling pathway

作者全名："Huang, Hao; Wang, Jian; Hussain, Shaik Althaf; Gangireddygari, Venkata Subba Reddy; Fan, Yingying"

作者地址："[Huang, Hao] Chongqing Med Univ, Affiliated Hosp 1, Dept Cardiothorac, Chongqing, Peoples R China; [Wang, Jian] Air Force Mil Med Univ, Affiliated Hosp 1, Dept Otolaryngol Head & Neck Surg, Xian, Peoples R China; [Hussain, Shaik Althaf] King Saud Univ, Coll Sci, Dept Zool, Riyadh, Saudi Arabia; [Gangireddygari, Venkata Subba Reddy] Rural Dev Adm, Natl Inst Hort & Herbal Sci, Plant Virus Res Hort & Herbal Crop Environm Div, Wonju, South Korea; [Fan, Yingying] Honghui Hosp, Dept Anesthesiol, Xian, Peoples R China; [Fan, Yingying] Honghui Hosp, Dept Anesthesiol, Xian 710068, Peoples R China"

通信作者："Fan, YY (通讯作者)，Honghui Hosp, Dept Anesthesiol, Xian 710068, Peoples R China."

来源：ENVIRONMENTAL TOXICOLOGY

ESI学科分类：ENVIRONMENT/ECOLOGY

WOS号：WOS:000981902800001

JCR分区：Q1

影响因子：4.4

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：antioxidant; gossypin; inflammation; lung inflammation; NF-kappa B signaling pathway

摘要："Acute Lung Injury (ALI) is a critical medical condition that induces the injury into the lung tissue, resulting in decreased the oxygen levels in the circulation and finally causes the respiratory failure. In this study, we try to made effort for scrutinized the preventive effect of gossypin against lipopolysaccharide (LPS) induced lung inflammation and explore the underlying mechanism. LPS (7.5 mg/kg) was used for induction the lung inflammation in the rats and rats were received the oral administration of gossypin (5, 10 and 15 mg/kg). The wet to dry weight lung ratio and lung index were estimated. The bronchoalveolar lavage fluid (BALF) were collected to determination the inflammatory cells, total protein, macrophages and neutrophils. ELISA kits were used for the estimation of antioxidant, inflammatory cytokines, inflammatory parameters, nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) parameters. Finally, we used the lung tissue for scrutinize the alteration in the lung histopathology. Gossypin treatment significantly (p < .001) reduced the W/D ratio of lung tissue and lung index. Gossypin significantly (p < .001) decreased the total cells, neutrophils, macrophages and total protein in BALF. It is also altered the level of inflammatory cytokines, antioxidant and inflammatory parameters, respectively. Gossypin improved the level of Nrf2 and HO-1 at dose dependent manner. Gossypin treatment remarkably enhance the ALI severity via balancing the structural integrity of lung tissue, decrease the thickness of the alveolar wall, decline the pulmonary interstitial edema, and number of inflammatory cells in the lung tissue. Gossypin is a promising agent for the treatment of LPS induced lung inflammation via altering Nrf2/HO-1 and NF-?B."

基金机构："King Saud University, Riyadh, Saudi Arabia [RSP2023R371]"

基金资助正文："ACKNOWLEDGEMENT The authors extend their appreciation to the Researchers SUpporting program for funding this work through Researchs Supporting Project number (RSP2023R371), King Saud University, Riyadh, Saudi Arabia."