Ghrelin improves endothelial function and reduces blood pressure in Ang II-induced hypertensive mice: Role of AMPK

作者全名："Deng, Juan; Hu, Yuan; Zhang, Yindi; Yu, Fadong"

作者地址："[Deng, Juan] Peoples Hosp Chongqing Liang Jiang New Area, Dept Endocrinol, Chongqing, Peoples R China; [Hu, Yuan] Songshan Gen Hosp, Dept Endocrinol, Chongqing, Peoples R China; [Zhang, Yindi; Yu, Fadong] Chongqing Med Univ, Affiliated Hosp 3, Dept Endocrinol, Chongqing 401120, Peoples R China"

通信作者："Yu, FD (通讯作者)，Chongqing Med Univ, Affiliated Hosp 3, Dept Endocrinol, Chongqing 401120, Peoples R China."

来源：CLINICAL AND EXPERIMENTAL HYPERTENSION

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:000982421200001

JCR分区：Q3

影响因子：1.5

年份：2023

卷号：45

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Ghrelin; endothelial function; hypertension; AMPK; oxidative stress

摘要："Background Endothelial dysfunction is a major pathophysiology observed in hypertension. Ghrelin, a key regulator of metabolism, has been shown to play protective roles in cardiovascular system. However, whether it has the effect of improving endothelial function and lowering blood pressure in Ang II-induced hypertensive mice remains unclear. Methods In this study, hypertension was induced by continuous infusion of Ang II with a subcutaneous osmotic pumps and ghrelin (30 mu g/kg/day) was intraperitoneal injection for 4 weeks. Acetylcholine-induced endothelium-dependent relaxation in aortae was measured on wire myograph and superoxide production in mouse aortae was assessed by fluorescence imaging. Results We found that ghrelin had protective effects on Ang II-induced hypertension by inhibiting oxidative stress, increasing NO production, improving endothelial function, and lowering blood pressure. Furthermore, ghrelin activated AMPK signaling in Ang II-induced hypertension, leading to inhibition of oxidative stress. Compound C, a specific inhibitor of AMPK, reversed the protective effects of ghrelin on the reduction of oxidative stress, the improvement of endothelial function and the reduction of blood pressure. Conclusions our findings indicated that ghrelin protected against Ang II-induced hypertension by improving endothelial function and lowering blood pressure partly through activating AMPK signaling. Thus, ghrelin may be a valuable therapeutic strategy for hypertension."

基金机构：Chongqing Science and Health Joint Medical Research Project [2022MSXM133]; Natural Science Foundation of Chongqing [CSTB2022NSCQ-MSX1522]

基金资助正文：The work was supported in part by grants from Chongqing Science and Health Joint Medical Research Project (2022MSXM133) and Natural Science Foundation of Chongqing (CSTB2022NSCQ-MSX1522).