Effects of Curcumin on Axon Growth and Myelin Sheath Formation in an In Vitro Model

作者全名："Yang, Luchen; Ren, Zhengju; Song, Pan; Liu, Zhenghuan; Peng, Zhufeng; Zhou, Jing; Dong, Qiang"

作者地址："[Yang, Luchen; Ren, Zhengju; Song, Pan; Liu, Zhenghuan; Peng, Zhufeng; Zhou, Jing; Dong, Qiang] Sichuan Univ, West China Hosp, Inst Urol, Dept Urol, Chengdu, Sichuan, Peoples R China; [Ren, Zhengju] Chongqing Med Univ, Affiliated Hosp 2, Dept Urol, Chongqing 400010, Peoples R China"

通信作者："Dong, Q (通讯作者)，Sichuan Univ, West China Hosp, Inst Urol, Dept Urol, Chengdu, Sichuan, Peoples R China."

来源：NEUROCHEMICAL RESEARCH

ESI学科分类：NEUROSCIENCE & BEHAVIOR

WOS号：WOS:000982722900001

JCR分区：Q2

影响因子：3.7

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：Nerve injuries; Curcumin; Axons; Schwann cells; Myelination

摘要："Although the beneficial effects of curcumin, extracted from rhizomes of the ginger family genus Curcuma, on the repair and regeneration of nerves have been evaluated in vitro, there are few studies concerning its effects on axon myelination. Here, we used pheochromocytoma cells as an in vitro model of peripheral nerves. Pheochromocytoma cells were cultured alone or cocultured with Schwann cells and treated with increasing concentrations of curcumin. Cell growth was observed, and the expression levels of growth-associated protein 43 (GAP-43), microtubule-associated protein 2 (MAP-2), myelin basic protein (MBP), myelin protein zero (MPZ), Krox-20, and octamer binding factor 6 (Oct-6) were quantified. We found a significant increase in expression of all six proteins following curcumin treatment, with a corresponding increase in the levels of MBP, MPZ, Krox-20, and Oct-6 mRNA. Upregulation was greater with increasing curcumin concentration, showing a concentration-dependent effect. The results suggested that curcumin can promote the growth of axons by upregulating the expression of GAP-43 and MAP-2, stimulate synthesis and secretion of myelin-related proteins, and facilitate formation of the myelin sheath in axons by upregulating the expression of Krox-20 and Oct-6. Therefore, curcumin could be widely applied in future strategies for the treatment of nerve injuries."

基金机构：135 project of West China Hospital of Sichuan University [ZY2016104]

基金资助正文：This work was funded by the 135 project of West China Hospital of Sichuan University (ZY2016104).