"Analysis of cytokine levels, cytological findings, and MP-DNA level in bronchoalveolar lavage fluid of children with Mycoplasma pneumoniae pneumonia"

作者全名："Deng, Fang; Cao, Huiling; Liang, Xiaohua; Li, Qubei; Yang, Yang; Zhao, Zhihua; Tan, Junjie; Fu, Guo; Shu, Chang"

作者地址："[Deng, Fang; Li, Qubei; Yang, Yang; Zhao, Zhihua; Shu, Chang] Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders, Minist Educ, Chongqing Key Lab Pediat,China Int Sci & Technol C, Chongqing, Peoples R China; [Deng, Fang] North Sichuan Med Coll, Dept Pediat, Affiliated Hosp, Nanchong, Peoples R China; [Cao, Huiling] Chongqing Med Univ, Dept Neonatol, Childrens Hosp, Chongqing, Peoples R China; [Liang, Xiaohua] Chongqing Med Univ, Dept Clin Epidemiol & Biostat, Childrens Hosp, Chongqing, Peoples R China; [Tan, Junjie; Fu, Guo] Chongqing Med Univ, Clin Mol Med Ctr, Childrens Hosp, Chongqing, Peoples R China; [Shu, Chang] 136 Zhongshan Er Rd,Yuzhong Dist, Chongqing 400014, Peoples R China"

通信作者："Shu, C (通讯作者)，136 Zhongshan Er Rd,Yuzhong Dist, Chongqing 400014, Peoples R China."

来源：IMMUNITY INFLAMMATION AND DISEASE

ESI学科分类：IMMUNOLOGY

WOS号：WOS:000983126200001

JCR分区：Q3

影响因子：3.1

年份：2023

卷号：11

期号：5

开始页：　

结束页：　

文献类型：Article

关键词：bronchoalveolar lavage fluid; cytokines; cytology; DNA; Mycoplasma pneumoniae pneumonia

摘要："BackgroundThe present study was conducted to determine the inflammatory response in the lungs of children with Mycoplasma pneumoniae pneumonia (MPP). MethodsThis study retrospectively analyzed cytokine levels, cytological findings, and M. pneumoniae (MP)-DNA level in the bronchoalveolar lavage fluid (BALF) of 96 children with MPP. The study utilized Spearman's correlation method to evaluate the contribution of BALF and blood parameters in MPP children. Results(1) A total of 96 MPP children were classified into the Low MP-DNA MPP group (BALF MP-DNA <= 10(5) copies/mL) and the High MP-DNA MPP group (BALF MP-DNA > 10(5)copies/mL); the Non-fever MPP group (no fever during the entire course of pneumonia) and the Fever MPP group; the Defervescence MPP group (fever had subsided at the time of bronchoscopy) and the Fervescence MPP group; and the Mild MPP group and the Severe MPP group. (2) The High MP-DNA MPP, Fever MPP, Fervescence MPP, and Severe MPP groups had higher levels of interleukin (IL)-6, IL-10, and tumor necrosis factor-alpha (TNF-alpha) in their BALF (all p < .05). (3) The proportions of neutrophils and macrophages in the BALF of the High MP-DNA MPP and Fever MPP groups increased and decreased, respectively (all p < .05). (4) In the BALF of MPP children, MP-DNA, IL-6, IL-10, TNF-alpha, and interferon gamma (IFN-gamma) levels positively correlated with neutrophil proportion while negatively correlated with macrophage proportion (all p < .05). (5) The MP-DNA, IL-6, IL-10, TNF-alpha, and IFN-gamma levels in the BALF of MPP children were positively correlated with the levels of C-reactive protein, procalcitonin, lactic dehydrogenase, fibrinogen, and d-dimer, while they were negatively correlated with the albumin level (all p < .05). ConclusionsIn children with MPP, the pulmonary inflammatory immune response was stronger in the High MP-DNA MPP, Fever MPP, Fervescence MPP, and Severe MPP groups. The relationship between pulmonary cytokine levels, MP-DNA load, and serum inflammatory parameters were found to be weak."

基金机构：Special scientific and technological innovation projects for social undertakingsand people's livelihood protection [cstc2017shmsA130032]; Chongqing High-end Medical Talent Cultivation Project for Young and Middle-aged People [2020-145]

基金资助正文："Special scientific and technological innovation projects for social undertakingsand people's livelihood protection, Grant/Award Number:cstc2017shmsA130032; Chongqing High-end Medical Talent Cultivation Project for Young and Middle-aged People (the 6thBatch), Grant/Award Number: 2020-145"