ADAR1 is a prognostic biomarker and is correlated with immune infiltration in lung adenocarcinoma

作者全名："Yang, Wendi; Chen, Kehong; Yu, Qian; Liao, Rongxin; He, Hengqiu; Peng, Yuan; Yang, Zhenzhou; Zhang, Xiaoyue"

作者地址："[Yang, Wendi; Chen, Kehong; Yu, Qian; Liao, Rongxin; He, Hengqiu; Peng, Yuan; Yang, Zhenzhou; Zhang, Xiaoyue] Chongqing Med Univ, Dept Canc Ctr, Affiliated Hosp 2, Chongqing, Peoples R China; [Yang, Zhenzhou; Zhang, Xiaoyue] Chongqing Med Univ, Dept Canc Ctr, Affiliated Hosp 2, Chongqing 400010, Peoples R China"

通信作者："Yang, ZZ; Zhang, XY (通讯作者)，Chongqing Med Univ, Dept Canc Ctr, Affiliated Hosp 2, Chongqing 400010, Peoples R China."

来源：CANCER MEDICINE

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000985751400001

JCR分区：Q2

影响因子：2.9

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：adenosine deaminase 1; lung adenocarcinoma; lymph node metastasis; tumor microenvironment

摘要："Background Lung adenocarcinoma (LUAD) is a common subtype of non-small cell lung cancer with high morbidity and mortality rates and is usually detected at advanced stages because of the early onset of metastasis. Adenosine deaminase RNA-specific 1 (ADAR1) is an RNA editing enzyme that catalyzes the important physiological process of adenosine-to-inosine editing and has been shown to participate in the progression of LUAD. Increasing evidence has suggested that immune infiltration of the tumor immune microenvironment has prognostic value for most human solid organ malignancies; however, much is unknown about the functions of ADAR1.Methods The expression of ADAR1 was analyzed in The Cancer Genome Atlas -LUAD database and validated in our LUAD cohort. To assess the prognostic value of ADAR1, Kaplan-Meier survival analyses and Cox regression analyses were carried out in LUAD cohorts. The association between ADAR1 and LUAD immune infiltrates via analyses of cell-type identification by estimating relative subsets of known RNA transcripts. Furthermore, multiplex immunohistochemistry was used to confirm the relationship between ADAR1 expression and immune cells in the present cohort of patients with LUAD.Results ADAR1 was highly expressed in LUAD tissues and closely correlated with lymph node metastasis (LNM) (p < 0.01), advanced tumor stage (p < 0.05), and poor patient prognosis (p < 0.01), thus indicating that increased ADAR1 contributed to the progression of LUAD. LUAD with high ADAR1 expression can metastasize to lymph nodes that express more ADAR1 than the primary lesion. In addition, M0 macrophages and M2 macrophages increased and CD4(+)T cells decreased in LUAD tissues with high ADAR1 expression. And the expression of ADAR1 in lymph node metastases was negatively correlated with the contents of CD4(+)T cells (p = 0.0017) and M1 macrophages (p = 0.0037).Conclusion The findings of our study suggested that ADAR1 may be useful in predicting prognosis and LNM in LUAD, and may serve as a promising immune-related molecular target for LUAD patients."

基金机构：Chongqing Postdoctoral Science Foundation [cstc2019jcyj-bshX0056]; Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University [kryc-yq-2221]; National Natural Science Foundation of China [82002448]; Project of Chongqing Technology Innovation and Application Development [CSTC2021jscx-gksb-N0022]

基金资助正文："Chongqing Postdoctoral Science Foundation, Grant/Award Number: cstc2019jcyj-bshX0056; Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University, Grant/Award Number: kryc-yq-2221; National Natural Science Foundation of China, Grant/Award Number: 82002448; the Project of Chongqing Technology Innovation and Application Development, Grant/Award Number: CSTC2021jscx-gksb-N0022"