Neutrophil extracellular traps promote bronchopulmonary dysplasia-like injury in neonatal mice via the WNT/beta-catenin pathway

作者全名："Sun, Liujuan; Zhang, Meiyu; Jiang, Jin; Liu, Wanjiao; Zhao, Wenhao; Li, Fang"

作者地址："[Sun, Liujuan; Zhang, Meiyu; Jiang, Jin; Liu, Wanjiao; Zhao, Wenhao; Li, Fang] Chongqing Med Univ, Dept Neonatal Diag & Treatment Ctr, Minist Educ, Key Lab Child Dev & Disorders,Childrens Hosp, Chongqing, Peoples R China; [Sun, Liujuan; Zhang, Meiyu; Jiang, Jin; Liu, Wanjiao; Zhao, Wenhao; Li, Fang] Natl Clin Res Ctr Child Hlth & Disorders, Chongqing, Peoples R China; [Sun, Liujuan; Zhang, Meiyu; Jiang, Jin; Liu, Wanjiao; Zhao, Wenhao; Li, Fang] China Int Sci & Technol Cooperat Base Child Dev &, Chongqing, Peoples R China; [Sun, Liujuan; Zhang, Meiyu; Jiang, Jin; Liu, Wanjiao; Zhao, Wenhao; Li, Fang] Chongqing Key Lab Pediat, Chongqing, Peoples R China"

通信作者："Li, F (通讯作者)，Chongqing Med Univ, Dept Neonatal Diag & Treatment Ctr, Minist Educ, Key Lab Child Dev & Disorders,Childrens Hosp, Chongqing, Peoples R China.; Li, F (通讯作者)，Natl Clin Res Ctr Child Hlth & Disorders, Chongqing, Peoples R China.; Li, F (通讯作者)，China Int Sci & Technol Cooperat Base Child Dev &, Chongqing, Peoples R China.; Li, F (通讯作者)，Chongqing Key Lab Pediat, Chongqing, Peoples R China."

来源：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY

ESI学科分类：MICROBIOLOGY

WOS号：WOS:000986540700001

JCR分区：Q1

影响因子：4.6

年份：2023

卷号：13

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：bronchopulmonary dysplasia; neutrophil extracellular traps; hyperoxia; bronchopulmonary dysplasia-like injury; Wnt; beta-catenin pathway

摘要："Background: Bronchopulmonary dysplasia (BPD) is one of the most common and severe chronic diseases in preterm infants. Premature infants are susceptible to BPD due to immature lungs and adverse perinatal episodes of infection, hyperoxia, and mechanical ventilation. Methods: Neutrophils are the first line of host defence, and the release of neutrophil extracellular traps (NETs) is an important strategy to immobilize and kill invading microorganisms. This study examined whether NETs were associated with BPD in preterm infants and contributed to hyperoxia-induced lung injury in neonatal mice via the WNT/beta-catenin pathway. Results: In this study, we found that preterm infants with BPD had higher levels of NETs in their tracheal aspirates than those without BPD. Neonatal mice treated with NETs after birth exhibited BPD-like changes in their lungs. Furthermore, the levels of Aquaporin 5 (AQP5) and surfactant-associated protein C (SPC), which represent alveolar differentiation and development, were significantly lower than those in the controls. The WNT/beta-catenin pathway is one of the most wellknown signalling pathways involved in lung growth. We found that the expression of the target genes c-MYC, cyclin D, and vascular endothelial growth factor (VEGF) and the important proteins WNT3a and beta-catenin significantly decreased. Moreover, heparin, which is a NET inhibitor, attenuated changes in gene and protein expression, thereby attenuating BPD-like changes. Discussion: This finding indicates that NETs are associated with BPD and can induce BPD-like changes in neonatal mice via the WNT/beta-catenin pathway."

基金机构：National Key Research and Development Program of China [2022YFC2704801]; Natural Science Foundation of Chongqing [cstc2021jcyj-msxmX0257]; Science and health key project of Chongqing Health Commission [2023ZDXM024]

基金资助正文：This work was supported by the National Key Research and Development Program of China (2022YFC2704801) and the Natural Science Foundation of Chongqing (cstc2021jcyj-msxmX0257). Science and health key project of Chongqing Health Commission (2023ZDXM024).