Electrochemiluminescence imaging of a membrane carcinoembryonic antigen at single tissue sections

作者全名："Shi, Junwei; Han, Dongni; Feng, Zengyu; Jiang, Dechen; Jiang, Depeng"

作者地址："[Shi, Junwei; Jiang, Depeng] Chongqing Med Univ, Affiliated Hosp 2, Dept Resp Med, Chongqing 400010, Peoples R China; [Han, Dongni; Jiang, Dechen] Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210023, Jiangsu, Peoples R China; [Feng, Zengyu] Zhejiang Univ, Affiliated Hosp 2, Sch Med, Dept Gen Surg, Hangzhou 310009, Peoples R China"

通信作者："Jiang, DP (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Dept Resp Med, Chongqing 400010, Peoples R China."

来源：ANALYST

ESI学科分类：CHEMISTRY

WOS号：WOS:000987173800001

JCR分区：Q2

影响因子：3.6

年份：2023

卷号：148

期号：11

开始页：2511

结束页：2517

文献类型：Article

关键词：　

摘要："Histopathological molecular testing of tissue sections is an essential step in tumor diagnosis; however, the commonly used immunohistochemical methods have problems such as low specificity and the subjective bias of the observer. Here, we report an electrochemiluminescence (ECL) imaging method to detect a membrane carcinoembryonic antigen (CEA) at the single tissue sections of cancer patients. By permeabilizing the tissue attached to a glassy carbon electrode, Ru(bpy)(3)(2+) tagged at the membrane CEA of the tissue could electrochemically react with TPrA in solution to emit ECL that has near-zero background and an extremely high signal-to-background ratio. Using the established ECL method, the expression differences and distribution characteristics of the CEA protein in the carcinoma and paracancerous tissues of pancreatic ductal carcinoma (PDAC) and lung adenocarcinoma (LUAD) patients are investigated. The images reveal that CEA proteins are mostly distributed in the acini and surrounding areas both in PDAC and LUAD tissues. Therefore, the presented approach could be able to provide a new molecular recognition method for the diagnosis of adenocarcinoma and other tumors."

基金机构：National Natural Science Foundation of China [22025403]; Chongqing Natural Science Foundation of China [cstc2022ycjh-bgzxm0051]; Postgraduate Research & Practice Innovation Program of Jiangsu Province [KYCX22_0101]

基金资助正文："This work was supported by the National Natural Science Foundation of China (no. 22025403), Chongqing Natural Science Foundation of China (no. cstc2022ycjh-bgzxm0051) and Postgraduate Research & Practice Innovation Program of Jiangsu Province (no. KYCX22_0101)."