The development of nasal polyps involves early middle meatus mucous remodeling via TGF-01 mediated PAI-1 reduction

作者全名："Liu, Yijun; Shu, Longlan; Jiang, Xiaocong; Zhang, Yue; Chen, Qian; Shen, Yang; Yang, Yucheng"

作者地址："[Liu, Yijun; Shu, Longlan; Jiang, Xiaocong; Zhang, Yue; Chen, Qian; Shen, Yang; Yang, Yucheng] Chongqing Med Univ, Affiliated Hosp 1, Dept Otorhinolaryngol, Chongqing, Peoples R China"

通信作者："Yang, YC (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Otorhinolaryngol, Chongqing, Peoples R China."

来源：BRAZILIAN JOURNAL OF OTORHINOLARYNGOLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000988724400001

JCR分区：Q2

影响因子：1.7

年份：2023

卷号：89

期号：3

开始页：366

结束页：373

文献类型：Article

关键词：Tissue remodeling; Fibrosis; Early nasal polyps; TGF-(31; Tissue culture in vitro

摘要："Objective: Our study aimed to elucidate the effect of PAI-1 (Plasminogen Activator Inhibitor-1) and t-PA (Tissue-type Plasminogen Activator) in tissue remodeling in nasal polyps patients. Methods: Samples were streamed as early Nasal Polyps (eNP, n = 10) and inferior tissue from the same patient, mature Nasal Polyps (mNP, n = 14), and Control group (n = 15), respectively. Immunohistochemistry and immunofluorescence were applied to detect localization. Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) and Western blot were used to measure different levels among three groups. The mNP tissue was cultured in vitro and treated with TGF-(31 (Transforming Growth Factor-beta 1) activator, TGF-(31 inhibitor (SB431542), and PAI-1 inhibitor (TM5275); then Western blot, qRT-PCR, and ELISA were used to assess changes. Results: The immunohistochemistry and immunofluorescence showed that PAI-1 expression decreased in eNP and mNP, mainly in epithelium and glands. The transcriptional expression and protein level of TGF-(31/t-PA/PAI-1/Collagen1 were lower in eNP than IT while mNP group demonstrated lower mRNA expression and protein level of TGF-(31/t-PA/PAI-1/Collagen1 than Control group. In mNP tissue culture in vitro, TGF-(31 activator elevated t-PA, PAI-1, and Collagen1 with higher release of PAI-1 and Collagen1 in supernatant, whereas SB431542 suppressed above reactions; TM5275 lowered transcriptional and protein level of Collagen1 in supernatant."

基金机构：National Natural Science Foundation of China [81970864]; Chongqing Middle and Youth Medical High-end Talent Studio Project [2]; Chongqing Talent Project [cstc2021ycjh-bgzxm0080]

基金资助正文："National Natural Science Foundation of China (grant no. 81970864) , the Chongqing Middle and Youth Medical High-end Talent Studio Project (grant no. Yu Wei, 2018, no. 2) , and the Chongqing Talent Project (grant no. cstc2021ycjh-bgzxm0080) ."