Scutellarin inhibits glioma cell proliferation by up-regulating miR-15a expression

作者全名："Du, Jiangfeng; Li, Ji; Tan, Jianjun; Yan, Yi"

作者地址："[Du, Jiangfeng; Yan, Yi] Chongqing Med Univ, Dept Neurosurg, Affiliated Hosp 1, 1 Med Coll Rd, Chongqing 400016, Peoples R China; [Du, Jiangfeng; Li, Ji] Chongqing Three Gorges Cent Hosp, Dept Neurosurg, 235 Beishan Ave, Chongqing 404000, Peoples R China; [Tan, Jianjun] Chongqing Three Gorges Cent Hosp, Dept Oncol, 235 Beishan Ave, Chongqing 404000, Peoples R China"

通信作者："Yan, Y (通讯作者)，Chongqing Med Univ, Dept Neurosurg, Affiliated Hosp 1, 1 Med Coll Rd, Chongqing 400016, Peoples R China."

来源：AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000989754300014

JCR分区：Q3

影响因子：1.7

年份：2023

卷号：15

期号：3

开始页：2156

结束页：2163

文献类型：Article

关键词：Scutellarin; microRNA-15a; glioma cell; proliferation; apoptosis; invasion

摘要："Objective: To investigate the effect of scutellarin on the proliferation of glioma cells through microRNA (miR)-15a. Methods: Human glioma cell line T98G was cultured in vitro and divided into control group (without treat-ment), scutellarin group (with 10, 20, 40, 80, 160 pg/mL scutellarin, respectively), miR-15a negative control group (transfected with negative control-miR-15a + 80 pg/mL scutellarin) and miR-15a inhibitor group (transfected with miR-15a siRNA + 80 pg/mL scutellarin). The proliferation of T98G cells was detected by cell counting kit-8 (CCK-8), and the expression of miR-15a in T98G cells was detected by real-time fluorescence quantitative PCR (qRT-PCR). The apoptosis of T98G cells was assessed by flow cytometry, and the invasion of T98G cells was compared by Transwell method. The levels of proliferating cell nuclear antigen (PCNA), Bcl-2 related X protein (Bax) and matrix metalloproteinase 9 (MMP-9) in T98G cells were detected by Western blot (WB). Results: Compared with that in the control group, the OD value of T98G cells in scutellarin group was significantly lower (P<0.05), with the increase of scutellarin concentration, the OD value of T98G cells decreased in turn, and 80 pg/mL was used as the optimal concentration of scutellarin to treat T98G cells for subsequent experiments. Compared with those in the control group, the miR-15a expression, apoptosis rate and Bax protein expression in T98G cells of scutellarin group were higher (P<0.05), and the OD value, number of invasive cells, PCNA and MMP-9 protein levels were lower (P<0.05). Compared with scutellarin group and miR-15a negative control group, the miR-15a expression, apoptosis rate and Bax protein expression in T98G cells of miR-15a inhibitor group were lower (P<0.05), and the OD value, number of invasive cells, PCNA and MMP-9 protein levels were higher (P<0.05). Conclusions: Scutellarin can inhibit the prolif-eration, invasion and induce the apoptosis of glioma cells, which may be mediated by up-regulating the expression of miR-15a."

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