Single-cell and spatial transcriptomics: Advances in heart development and disease applications

作者全名："Long, Xianglin; Yuan, Xin; Du, Jianlin"

作者地址："[Long, Xianglin; Du, Jianlin] Chongqing Med Univ, Dept Cardiol, Affiliated Hosp 2, 74 Lin Jiang Rd, Chongqing 400010, Peoples R China; [Yuan, Xin] Chongqing Med Univ, Dept Nephrol, Affiliated Hosp 2, 74 Lin Jiang Rd, Chongqing 400010, Peoples R China"

通信作者："Du, JL (通讯作者)，Chongqing Med Univ, Dept Cardiol, Affiliated Hosp 2, 74 Lin Jiang Rd, Chongqing 400010, Peoples R China.; Yuan, X (通讯作者)，Chongqing Med Univ, Dept Nephrol, Affiliated Hosp 2, 74 Lin Jiang Rd, Chongqing 400010, Peoples R China."

来源：COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000990756400001

JCR分区：Q2

影响因子：4.4

年份：2023

卷号：21

期号：　

开始页：2717

结束页：2731

文献类型：Review

关键词：Single-cell RNA sequencing; Spatial transcriptomics; Cardiogenesis; Cardiology; Precision medicine

摘要："Current transcriptomics technologies, including bulk RNA-seq, single-cell RNA sequencing (scRNA-seq), single-nucleus RNA-sequencing (snRNA-seq), and spatial transcriptomics (ST), provide novel insights into the spatial and temporal dynamics of gene expression during cardiac development and disease processes. Cardiac development is a highly sophisticated process involving the regulation of numerous key genes and signaling pathways at specific anatomical sites and developmental stages. Exploring the cell biological mechanisms involved in cardiogenesis also contributes to congenital heart disease research. Meanwhile, the severity of distinct heart diseases, such as coronary heart disease, valvular disease, cardiomyopathy, and heart failure, is associated with cellular transcriptional heterogeneity and phenotypic alteration. Integrating transcriptomic technologies in the clinical diagnosis and treatment of heart diseases will aid in advancing precision medicine. In this review, we summarize applications of scRNA-seq and ST in the cardiac field, including organogenesis and clinical diseases, and provide insights into the promise of single-cell and spatial transcriptomics in translational research and precision medicine.(c) 2023 The Authors. Published by Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology. This is an open access article under the CC BY-NC-ND license (http://creative-commons.org/licenses/by-nc-nd/4.0/)."

基金机构：National Natural Science Foundation of China (NSFC) [82270281]; Natural Science Foundation of Chongqing Science and Technology Commission [cstc2020jcyj-msxmX0210]; Future Medicine Youth Innovation Team Development Support Program of Chongqing Medical University [W0133]; Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University; High-end Medical Talents Project of Middle-aged and Young People in Chongqing

基金资助正文：This work was supported by grants from the National Natural Science Foundation of China (NSFC) (82270281); Natural Science Foundation of Chongqing Science and Technology Commission (cstc2020jcyj-msxmX0210); Future Medicine Youth Innovation Team Development Support Program of Chongqing Medical University (W0133); Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University; High-end Medical Talents Project of Middle-aged and Young People in Chongqing (JianlinDu [2022]).