Conductive nano-gold self-assembled MXene@hemin with high catalytic activity achieved by strong metal-support interactions: A powerful nanozyme for development of electrochemical aptasensor in tuberculosis diagnosis

作者全名："Chen, Yuhan; Gu, Yue; Yi, Xiaojuan; Huang, He; Li, Yishi; Yang, Bijun; Guo, Shuliang; Bai, Lijuan"

作者地址："[Chen, Yuhan; Huang, He; Li, Yishi; Yang, Bijun; Guo, Shuliang] Chongqing Med Univ, Dept Resp & Crit Care Med, Affiliated Hosp 1, Chongqing 400016, Peoples R China; [Gu, Yue] Chongqing Med Univ, Dept Endocrinol, Affiliated Hosp 1, Chongqing 400016, Peoples R China; [Yi, Xiaojuan; Bai, Lijuan] Chongqing Med Univ, Coll Pharm, Chongqing Res Ctr Pharmaceut Engn, Chongqing 400016, Peoples R China"

通信作者："Guo, SL (通讯作者)，Chongqing Med Univ, Dept Resp & Crit Care Med, Affiliated Hosp 1, Chongqing 400016, Peoples R China.; Bai, LJ (通讯作者)，Chongqing Med Univ, Coll Pharm, Chongqing Res Ctr Pharmaceut Engn, Chongqing 400016, Peoples R China."

来源：CHEMICAL ENGINEERING JOURNAL

ESI学科分类：ENGINEERING

WOS号：WOS:000990799400001

JCR分区：Q1

影响因子：13.3

年份：2023

卷号：466

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Nanozymes; Metal -support interaction; Tuberculosis diagnosis; Electrochemical biosensor

摘要："Despite multiple advances in tuberculosis (TB), there is still a lack of effective methods available for its diagnosis. To this end, we developed a novel nanozyme-mediated electrochemical aptasensor for TB diagnosis. We showed that hemin decorated with multi-layered MXene enhanced the metal-support interaction with nano-gold via charge transfer, resulting in excellent peroxidase-like activity aside from the highly conductive feature. The Km value of hemin functionalized MXene decorated with nano-gold (MXene@hemin-Au) was 0.37 mM and 0.23 mM for H2O2 and TMB, respectively, which was smaller to that of MXene@hemin (4.01 mM and 0.39 mM) or horseradish peroxidase (3.7 mM and 0.434 mM), indicating its high affinity to both substrates, showing improved stability in harsh conditions and excellent biocompatibility. Given these advantages, an ultrasensitive detection method was established based on a typical sandwich format using a combination of specific Myco-bacterium tuberculosis (MTB) ESAT-6 antigen aptamers with MXene@hemin-Au as probes for signal amplification and gold nanoparticles supported nitrogen-doped carbon nanotube (N-CNTs-Au) as a sensing platform. It exhibited a wide detection linear for ESAT-6 antigen from 100 fg mL-1 to 50 ng mL-1, with limit of detection and limit of quantification of 2.36 fg mL-1 and 7.87 fg mL-1, respectively. For its diagnostic application, of note, the proposed method outperformed diagnostic performance for TB in contract to a commercial ELISA method in terms of specificity (100% vs. 20.59%) and area under curve (0.987 vs. 0.596), verifying its great potential in clinical practice."

基金机构："National Natural Science Foundation of China [82072378, 81601856]; National Major Science and Technology Projects of China [2018ZX10302302003]; Chongqing Talents: Exceptional Young Talents Project, China [CQYC202005015, cstc2021ycjh-bgzxm0328]; Program for Youth Innovation in Future Medicine, Chongqing Medical University, China [YXY2019XSGG4]; Funds for High Level Young Science and Technology Talent Cultivation Plan in Chongqing Medical University, China (2019); Discipline Talent Training Program of College of Pharmacy in Chongqing Medical University, China [YXY2021BSH04, cstc2014kjrc-qnrc00004]; Funds for Young Science and Technology Talent Cultivation Plan of Chongqing City, China [499PYJJ2021-10]; Cultivation Foundation of the First Affiliated Hospital of Chongqing Medical University [W0119]; Program for Youth Innovation in Future Medicine, Chongqing Medical University [YS2019020]; Chongqing talents projects-Famous masters and teachers (Shuliang Guo), Ba Yu Scholar Program, China; [W0067]"

基金资助正文："This work is supported by National Natural Science Foundation of China (82072378, 81601856), the National Major Science and Technology Projects of China (2018ZX10302302003), Chongqing Talents: Exceptional Young Talents Project, China (CQYC202005015, cstc2021ycjh-bgzxm0328), Chongqing talents projects-Famous masters and teachers (Shuliang Guo), Ba Yu Scholar Program, China (YS2019020), Program for Youth Innovation in Future Medicine, Chongqing Medical University, China (W0067), Funds for High Level Young Science and Technology Talent Cultivation Plan in Chongqing Medical University, China (2019), Discipline Talent Training Program of College of Pharmacy in Chongqing Medical University, China (YXY2019XSGG4, YXY2021BSH04) and Funds for Young Science and Technology Talent Cultivation Plan of Chongqing City, China (cstc2014kjrc-qnrc00004), Cultivation Foundation of the First Affiliated Hospital of Chongqing Medical University (499PYJJ2021-10). Program for Youth Innovation in Future Medicine, Chongqing Medical University (W0119)."