The expression of interleukin-1 beta in patients with chronic hepatitis B treated with pegylated-interferon-alpha combined with tenofovir disoproxil fumarate and monotherapy

作者全名："Hu, Xiaoxia; Luo, Haiying; Tan, Guili; Li, Yadi; Qin, Bo"

作者地址："[Hu, Xiaoxia; Luo, Haiying; Tan, Guili; Li, Yadi; Qin, Bo] Chongqing Med Univ, Dept Infect Dis, Chongqing Key Lab Infect Dis & Parasit Dis, Affiliated Hosp 1, 1 Youyi Rd, Chongqing 400016, Peoples R China"

通信作者："Qin, B (通讯作者)，Chongqing Med Univ, Dept Infect Dis, Chongqing Key Lab Infect Dis & Parasit Dis, Affiliated Hosp 1, 1 Youyi Rd, Chongqing 400016, Peoples R China."

来源：BMC GASTROENTEROLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000991005800001

JCR分区：Q3

影响因子：2.4

年份：2023

卷号：23

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Pegylated interferon alpha; Tenofovir disoproxil fumarate; Chronic hepatitis B; Early response; Interleukin-1 beta

摘要："BackgroundAnti-hepatitis B virus (HBV) treatment uses tenofovir disoproxil fumarate (TDF) along with Pegylated-interferon-alpha (Peg-IFN-alpha), which is more effective than TDF/Peg-IFN-alpha monotherapy. We have previously shown that interleukin-1beta (IL-1 beta) is related to the effectiveness of IFN-alpha treatment in chronic hepatitis B (CHB) patients. The aim was to investigate the expression of IL-1 beta in CHB patients treated with Peg-IFN-alpha combination with TDF and TDF/Peg-IFN-alpha monotherapy.MethodsHuh7 cells infected with HBV were stimulated by Peg-IFN-alpha and/or Tenofovir (TFV) for 24h. A single-center cohort study of prospective recruitment of CHB patients: untreated CHB (Group A), TDF combined with Peg-IFN-alpha therapy (Group B), Peg-IFN-alpha monotherapy (Group C), TDF monotherapy (Group D). Normal donors served as controls. The clinical datas and blood of patients were collected at 0, 12, and 24 weeks. According to the early response criteria, Group B and C were divided into two subgroups: the early response group (ERG) and the non-early response group (NERG). Stimulation of HBV-infected hepatoma cells with IL-1 beta to validate the antiviral activity of IL-1 beta. To test the blood sample, cell culture supernatant, and cell lysates and to assess the expression of IL-1 beta and HBV replication levels in various treatment protocols, Enzyme-Linked Immunosorbent Assay (ELISA) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) were used. SPSS 26.0 and GraphPad Prism 8.0.2 software were used for statistical analysis. P values < 0.05 was considered to be statistically significant.ResultsIn vitro experiments, Peg-IFN-alpha plus TFV treatment group expressed higher IL-1 beta and inhibited HBV more effectively than monotherapy. Finally, 162 cases were enrolled for observation (Group A (n = 45), Group B (n = 46), Group C (n = 39), and Group D (n = 32)), and normal donors (n = 20) were enrolled for control. The early virological response rates of Group B, C, and D were 58.7%, 51.3%, and 31.2%. At 24 weeks, IL-1 beta in Group B(P = 0.007) and C(P = 0.034) showed higher than at 0 week. In Group B, the IL-1 beta showed an upward trend at 12w and 24w in the ERG. IL-1 beta significantly reduced HBV replication levels in hepatoma cells.ConclusionThe increased expression of IL-1 beta may enhance the efficacy of TDF combined with Peg-IFN-alpha therapy in achieving an early response for CHB patients."

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