Significant elevated CXCL14 and decreased IL-39 levels in patients with tuberculosis

作者全名："Ding, Min; Wang, Hong-xu; Gao, Si-jia; Lai, Xiao-fei; Li, An-long; Bao, Jia-jia; Hosyanto, Felycia-Fernanda; Xu, Lei"

作者地址："[Gao, Si-jia; Li, An-long; Xu, Lei] Chongqing Med Univ, Sch Basic Med, Pathogen Biol Dept, Chongqing 400016, Peoples R China; [Ding, Min] Chongqing Med Univ, Affiliated Hosp 1, Dept Resp, Chongqing 400016, Peoples R China; [Wang, Hong-xu; Lai, Xiao-fei] Chongqing Med Univ, Affiliated Hosp 1, Dept Lab Med, Chongqing 400016, Peoples R China; [Bao, Jia-jia] First Peoples Hosp Jintang Cty, Hosp Acquired Infect Control Dept, Chengdu 610499, Peoples R China; [Hosyanto, Felycia-Fernanda] Chongqing Med Univ, Dept Clin Med, Chongqing 400016, Peoples R China"

通信作者："Xu, L (通讯作者)，Chongqing Med Univ, Sch Basic Med, Pathogen Biol Dept, Chongqing 400016, Peoples R China."

来源：OPEN LIFE SCIENCES

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000991895100001

JCR分区：Q3

影响因子：1.7

年份：2023

卷号：18

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：IL-39; CXCL14; tuberculosis; BCG; cytokine; biomarker

摘要："To explore the serum levels of IL-39, CXCL14, and IL-19 in patients with tuberculosis (TB) along with their clinical significances and their concentration changes in macrophages after Bacille Calmette-Guerin vaccine (BCG) or Mycobacterium tuberculosis (M. tb) H37Rv stimulation in vitro. The serum levels of IL-39, CXCL14, and IL-19 of 38 TB patients, and 20 healthy staff members were measured by enzyme-linked immunosorbent assay. Moreover, the levels of IL-19, CXCL14, and IL-39 in cultured THP-1 macrophages were detected at 12, 24, and 48 h after stimulation with BCG or M. tb H37Rv strains. It was found the serum level of IL-39 was significantly reduced and CXCL14 was remarkably elevated in TB patients. In vitro, at 48 h after stimulation, IL-39 level of cultured THP-1 macrophages in the H37Rv group was significantly lower than that in the BCG and control groups, and the CXCL14 level of cultured THP-1 macrophages in the H37Rv stimulation group was remarkably higher than that in the control group. Therefore, IL-39 and CXCL14 may be involved the pathogenesis of TB, and serum IL-39 and CXCL14 could potentially serve as a new biomarker of TB."

基金机构："Scientific and Technological Research Program of Chongqing Municipal Education Commission [KJQN201900417]; Chongqing Municipal Science and Technology Commission [cstc2017jcyjAX0409, cstc2021jcyj-msxmX0123]; Chongqing Science and Health Joint Project [2023MSXM049]; Program for Youth Innovation in Future Medicine, Chongqing Medical University [W0091]"

基金资助正文："This work was financially supported by Scientific and Technological Research Program of Chongqing Municipal Education Commission (no. KJQN201900417), Chongqing Municipal Science and Technology Commission (no. cstc2017jcyjAX0409 and no. cstc2021jcyj-msxmX0123), Chongqing Science and Health Joint Project (no. 2023MSXM049),and Program for Youth Innovation in Future Medicine, Chongqing Medical University(W0091)"