Aptamer functionalized cell membrane for brain and nerve cell sensing with high sensitivity and stability

作者全名："Wu, Hui; Meng, Zexuan; Wang, Jian; Yao, Guoqing; Yang, Lu; Zeng, Zhongyuan; She, Kepeng; Zhao, Shuang; Wang, Guixue; Zhang, Yuchan; Zang, Guangchao"

作者地址："[Wu, Hui; Meng, Zexuan; Wang, Jian; Yao, Guoqing; Yang, Lu; Zeng, Zhongyuan; She, Kepeng; Zhang, Yuchan; Zang, Guangchao] Chongqing Med Univ, Inst Life Sci, Management Ctr, Lab Tissue & Cell Biol, Chongqing 400016, Peoples R China; [Zhao, Shuang; Wang, Guixue] Chongqing Univ, Minist Educ, Key Lab Biorheol Sci & Technol, State & Local Joint Engn Lab Vasc Implants,Bioengn, Chongqing 400030, Peoples R China; [Zhao, Shuang; Wang, Guixue; Zang, Guangchao] Jinfeng Lab, Chongqing 401329, Peoples R China; [Wang, Jian; Zang, Guangchao] Chongqing Med Univ, Dept Pathophysiol, Chongqing, Peoples R China"

通信作者："Zhang, YC; Zang, GC (通讯作者)，Chongqing Med Univ, Inst Life Sci, Management Ctr, Lab Tissue & Cell Biol, Chongqing 400016, Peoples R China.; Wang, GX (通讯作者)，Chongqing Univ, Minist Educ, Key Lab Biorheol Sci & Technol, State & Local Joint Engn Lab Vasc Implants,Bioengn, Chongqing 400030, Peoples R China.; Wang, GX; Zang, GC (通讯作者)，Jinfeng Lab, Chongqing 401329, Peoples R China.; Zang, GC (通讯作者)，Chongqing Med Univ, Dept Pathophysiol, Chongqing, Peoples R China."

来源：BIOSENSORS & BIOELECTRONICS

ESI学科分类：CHEMISTRY

WOS号：WOS:000991902800001

JCR分区：Q1

影响因子：10.7

年份：2023

卷号：227

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Chitosan; Brain cell membrane; Aptamer cholesterol amphiphiles; Carbon fiber microelectrode; Dopamine

摘要："Accurate dopamine (DA) monitoring with high stability is essential for investigating the chemical basis of brain function and pathology. Electrochemical-based tissue-implantable carbon fiber electrodes (CFEs) show great potential in sensing the dynamics of neurochemicals at a sub-second timescale. However, their anti-fouling property, selectivity, and stability pose challenges. Here, we presented a novel strategy to enhance electrode biocompatibility and stability by modifying CFE with a chitosan (CS) film, brain cell membrane (M), and aptamer cholesterol amphiphiles (DNA-cho). We found that CFE was uniformly covered by a cicada-like membrane after being modified. Electrochemical characterizations indicated that DNA-cho-M-CS-CFE exhibited a wide linear range of DA concentration and showed high sensitivity, specificity, and stability. The electrode also presented excellent fouling resistance and biocompatibility. Moreover, the biosensor was used to detect DA in K+-induced brain slices and PC12 cells with a satisfactory stability and sensitivity and to prove that LPS treatment leads to the delayed and decreased release of DA. DNA-cho-M-CS-CFE showed excellent electrochemical performance and unique advantages for long-term in vivo sensing of living cells, thus providing a new feasible scheme for studying neurochemical kinetics and brain diseases."

基金机构："Natural Science Foundation of Chongqing [cstc2020jcyj-msxmX0330]; JinFeng Laboratory Foundation, Chongqing, China [jfkyjf202203001]; Project of Scientific Research and Innovative Experiment for College Student in Chongqing Medical University [SRIEP202128, SPIEP202167, S202010631016, CYS22333]; National Project of University Students Innovation and Entrepreneurship Training Program [LTMCMTS202109]; Chongqing Municipal Education Commission Foundation [LTMCMTS202106]; Project of Tutorial System of Medical Undergraduate in Lab Teaching & Management Center in Chongqing Medical University; [SRIEP202011]; [SRIEP202047]"

基金资助正文："This work was supported by grants the Natural Science Foundation of Chongqing (cstc2020jcyj-msxmX0330); the JinFeng Laboratory Foundation, Chongqing, China (jfkyjf202203001); the Project of Tutorial System of Medical Undergraduate in Lab Teaching & Management Center in Chongqing Medical University (LTMCMTS202109, LTMCMTS202106), the Project of Scientific Research and Innovative Experiment for College Student in Chongqing Medical University (SRIEP202011, SRIEP202047, SRIEP202128, SPIEP202167) , the National Project of University Students Innovation and Entrepreneurship Training Program (S202010631016), and Chongqing Municipal Education Commission Foundation (CYS22333)."