Effects of task-based rehabilitative training combined with PTEN/SOCS3 coinhibition promotes axon regeneration and upper extremity skilled motor function recovery after cervical spinal cord injury in adult mice

作者全名："Pan, Lu; Yi, Lingrong; Liu, Yuan; Liu, Li; Zhu, Ying; Zhong, Juan; Wang, Yunhang; Yin, Ying; Yu, Lehua; Tan, Botao; Yang, Ce"

作者地址："[Pan, Lu; Liu, Yuan; Wang, Yunhang; Yang, Ce] Army Med Univ, Dept Res, State Key Lab Trauma Burns & Combined Injury, Inst Surg, Chongqing 400042, Peoples R China; [Pan, Lu; Yi, Lingrong; Zhu, Ying; Zhong, Juan; Yin, Ying; Yu, Lehua; Tan, Botao] Chongqing Med Univ, Affiliated Hosp 2, Dept Rehabil Med, Chongqing 400010, Peoples R China; [Liu, Li] Chongqing Hosp Tradit Chinese Med, Dept Brain, Chongqing 400011, Peoples R China"

通信作者："Yang, C (通讯作者)，Army Med Univ, Dept Res, State Key Lab Trauma Burns & Combined Injury, Inst Surg, Chongqing 400042, Peoples R China.; Tan, BT (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Dept Rehabil Med, Chongqing 400010, Peoples R China."

来源：NEUROSCIENCE LETTERS

ESI学科分类：NEUROSCIENCE & BEHAVIOR

WOS号：WOS:000992852700001

JCR分区：Q3

影响因子：2.5

年份：2023

卷号：800

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Axon regeneration; Neuron-intrinsic growth ability; Task-based rehabilitative training; Skilled motor function; Spinal cord injury

摘要："Previous studies reported that the codeletion of PTEN and SOCS3 can greatly enhance the capacity of axon regeneration after central nervous system (CNS) injury. Moreover, the promotion of functional recovery can be improved by rehabilitative training under a use-dependent plasticity mechanism after CNS injury. However, few studies have reported the interaction between these mechanisms after spinal cord injury (SCI). Therefore, we investigated the combined effects of PTEN/SOCS3 coinhibition and rehabilitative training on axon regeneration and upper extremity motor functional improvement after cervical SCI in mice. In this study, we used RNA interference viruses to coinhibit PTEN and SOCS3 and induced a C5 crush injury on the side of preference. The injured upper extremity was trained by single pellet grasping for 4 weeks. We found that the coinjection of viruses significantly increased the expression of p-S6 and p-STAT in the cortex, reduced the dieback pattern of injured axons and promoted traced axon regeneration. More importantly, combination therapy further enhanced axon regeneration compared with PTEN/SOCS3 coinhibition alone. In behavioral tests, the motor performance of the mice in the PTEN/SOCS3 + Training group was better than that of the mice in the other groups. These results indicate that combining task-based rehabilitative training with PTEN/SOCS3 coinhibition further promotes axon regeneration and significant improvement in forelimb skilled motor function after cervical SCI. Our findings provide new therapeutic insights into SCI treatment."

基金机构："National Natural Science Foundation of China [81702221, 81772066]; Natural Science Foundation of Chongqing [cstc2018jcyjAX0180]; Open Project of the State Key Laboratory of Trauma, Burn and Combined Injury, Army Medical University [SKLKF202111]; Army Medical Center Talent Innovation Ability Training Program [2019CXJSB007]"

基金资助正文："This work was supported in part by the: National Natural Science Foundation of China (81702221, 81772066) , Natural Science Foundation of Chongqing (cstc2018jcyjAX0180) , the Open Project of the State Key Laboratory of Trauma, Burn and Combined Injury, Army Medical University (SKLKF202111) and the Army Medical Center Talent Innovation Ability Training Program (2019CXJSB007) ."