"Cordycepin Inhibits Pathological High Shear-Induced Platelet Aggregation, Activation, and Phosphatidylserine Exposure by Regulating the Phosphorylation of Akt Protein"

作者全名："Gao, Xuemei; Zhang, Tiancong; Huang, Xiaojing; Huan, Xuanrong; He, Cui; Li, Yuan"

作者地址："[Gao, Xuemei; Zhang, Tiancong; Huang, Xiaojing; Li, Yuan] Chongqing Med Univ, Cent Lab, Yong Chuan Hosp, Chongqing 402160, Peoples R China; [Huan, Xuanrong] Chongqing Med Univ, Yong Chuan Hosp, Dept Clin Lab, Chongqing 402160, Peoples R China; [He, Cui] Chongqing Med Univ, Yong Chuan Hosp, Dept Blood Transfus, Chongqing 402160, Peoples R China"

通信作者："Li, Y (通讯作者)，Chongqing Med Univ, Cent Lab, Yong Chuan Hosp, Chongqing 402160, Peoples R China."

来源：REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:000993014100001

JCR分区：Q4

影响因子：1.4

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：Akt phosphorylation; 3 '-Deoxyadenosine; Glycoprotein activation; Microfluidic chip system; Platelet cytotoxicity; Shear stress

摘要："Cordycepin can effectively inhibit adenosine diphosphate-induced platelet aggregation; however, the inhibitory effect on shear-induced platelet aggregation is not known. Pathological high shear stress is an important factor in inducing platelet aggregation in the human body. In order to further study the antithrombotic effect and mechanism of cordycepin, in this study, a microfluidic chip model was developed that not only can mimic stenotic vessels but also provide high shear stress needed in the experiment. The inhibitory effects of cordycepin on platelet aggregation, activation of P-selectin and glycoprotein IIb/IIIa, phosphatidyl serine exposure, glycoprotein Ib/von Willebrand factor binding, and Akt protein phosphorylation, induced by a pathological high shear rate (10,000 s(-1)), were studied. In addition, we also investigated the effect of cordycepin on adenosine diphosphate and arachinodic acid-induced platelet aggregation by turbidimetry. The results showed that cordycepin can inhibit platelet aggregation, P-selectin expression, glycoprotein IIb/IIIa activation, phosphatidylserine exposure, and Akt protein phosphorylation induced by a pathological high shear rate. It did not affect the combination of GPIb and vWF because the combination of CD14-staind platelets and CD41-staind platelets by von Willebrand factor was not inhibited by cordycepin. Under static conditions, cordycepin can inhibit adenosine diphosphate and arachinodic acid-induced platelet aggregation."

基金机构：Graduate Innovation Fund of Yongchuan Hospital; Joint Project of Chongqing Health Commission and Science and Technology Bureau [2023GDRC008]

基金资助正文："This work was supported by Graduate Innovation Fund of Yongchuan Hospital affiliated to Chongqing Medical University (YJSCX202204), and Joint Project of Chongqing Health Commission and Science and Technology Bureau (2023GDRC008)."