Transcranial Doppler Ultrasonography detection on cerebrovascular flow for evaluating neonatal hypoxic-ischemic encephalopathy modeling
作者全名："Liu, Jin-Xiang; Fang, Chang-Le; Zhang, Kun; Ma, Rui-Fang; Zhou, Hong-Su; Chen, Li; Wang, Qiu-Lin; Lu, Yu-Xuan; Wang, Ting-Hua; Xiong, Liu-Lin"
作者地址："[Liu, Jin-Xiang; Zhou, Hong-Su; Xiong, Liu-Lin] Zunyi Med Univ, Affiliated Hosp, Dept Anesthesiol, Zunyi, Guizhou, Peoples R China; [Liu, Jin-Xiang; Fang, Chang-Le; Ma, Rui-Fang; Wang, Ting-Hua] Kunming Med Univ, Lab Anim Dept, Kunming, Peoples R China; [Fang, Chang-Le; Lu, Yu-Xuan] Southwest Med Univ, Sch Anesthesiol, Luzhou, Peoples R China; [Zhang, Kun] Shantou Ultrason Instrument Res Inst Co Ltd, Shantou, Guangdong, Peoples R China; [Chen, Li; Wang, Ting-Hua] Sichuan Univ, West China Hosp, Inst Neurol Dis, Translat Neurosci Ctr, Chengdu, Peoples R China; [Wang, Qiu-Lin] Chongqing Med Univ, Dept Clin Med, Chongqing, Peoples R China; [Xiong, Liu-Lin] Kunming Univ Sci & Technol, Yunnan Key Lab Primate Biomed Res, Kunming, Peoples R China"
通信作者："Xiong, LL (通讯作者)，Zunyi Med Univ, Affiliated Hosp, Dept Anesthesiol, Zunyi, Guizhou, Peoples R China.; Wang, TH (通讯作者)，Kunming Med Univ, Lab Anim Dept, Kunming, Peoples R China.; Wang, TH (通讯作者)，Sichuan Univ, West China Hosp, Inst Neurol Dis, Translat Neurosci Ctr, Chengdu, Peoples R China.; Xiong, LL (通讯作者)，Kunming Univ Sci & Technol, Yunnan Key Lab Primate Biomed Res, Kunming, Peoples R China."
来源：FRONTIERS IN NEUROSCIENCE
ESI学科分类：NEUROSCIENCE & BEHAVIOR
WOS号：WOS:000993449000001
JCR分区：Q2
影响因子：3.2
年份：2023
卷号：17
期号：
开始页：
结束页：
文献类型：Article
关键词：Transcranial Doppler Ultrasonography; neonatal hypoxic-ischemic encephalopathy modeling; cerebrovascular flow; cerebral blood vessels; cerebral infarct
摘要："ObjectiveThis study aimed to investigate the feasibility of Transcranial Doppler Ultrasonography (TCD) in evaluating neonatal hypoxic-ischemic encephalopathy (NHIE) modeling through monitoring the alteration of cerebrovascular flow in neonatal hypoxic-ischemic (HI) rats. MethodsPostnatal 7-day-old Sprague Dawley (SD) rats were divided into the control group, HI group, and hypoxia (H) group. TCD was applied to assess the changes of cerebral blood vessels, cerebrovascular flow velocity, and heart rate (HR) in sagittal and coronal sections at 1, 2, 3, and 7 days after the operation. For accuracy, cerebral infarct of rats was examined by 2,3,5-Triphenyl tetrazolium chloride (TTC) staining and Nissl staining to simultaneously verify the establishment of NHIE modeling. ResultsCoronal and sagittal TCD scans revealed obvious alteration of cerebrovascular flow in main cerebral vessels. Obvious cerebrovascular back-flow was observed in anterior cerebral artery (ACA), basilar artery (BA), middle cerebral artery (MCA) of HI rats, along with accelerated cerebrovascular flows in the left internal carotid artery (ICA-L) and BA, decreased flows in right internal carotid artery (ICA-R) relative to those in the H and control groups. The alterations of cerebral blood flows in neonatal HI rats indicated successful ligation of right common carotid artery. Besides, TTC staining further validated the cerebral infarct was indeed caused due to ligation-induced insufficient blood supply. Damage to nervous tissues was also revealed by Nissl staining. ConclusionCerebral blood flow assessment by TCD in neonatal HI rats contributed to cerebrovascular abnormalities observed in a real-time and non-invasive way. The present study elicits the potentials to utilize TCD as an effective means for monitoring the progression of injury as well as NHIE modeling. The abnormal appearance of cerebral blood flow is also beneficial to the early warning and effective detection in clinical practice."
基金机构：Collaborative Innovation Center of Ministry of Education [2020-39]
基金资助正文："Funding This study was financially supported by National Natural Science Foundation of China (No. 82001604), Science and Technology Plan Project of Guizhou Province [Guizhou Science and Technology Foundation-KZ (2021) General 368], and Joint Fund of Zunyi Science and Technology Bureau-Affiliated Hospital of Zunyi Medical University (No. HZ2020250)."
