Particle Size-Controlled Oxygen Reduction and Evolution Reaction Nanocatalysts Regulate Ru(bpy)32+'s Dual-potential Electrochemiluminescence for Sandwich

作者全名："Wang, Shijun; Zhu, Shu; Kang, Ziili; Wang, Xiangxiu; Deng, Zixin; Hu, Kun; Hu, Jianjun; Liu, Xiancheng; Wang, Guixue; Zang, Guangchao; Zhang, Yuchan"

作者地址："[Wang, Shijun; Zhu, Shu; Kang, Ziili; Deng, Zixin; Hu, Kun; Zang, Guangchao; Zhang, Yuchan] Chongqing Med Univ, Inst Life Sci & Lab Tissue & Cell Biol, Lab Teaching Management Ctr, Chongqing 400016, Peoples R China; [Wang, Xiangxiu; Liu, Xiancheng; Wang, Guixue] Chongqing Univ, Key Lab Biorheol Sci & Technol, State & Local Joint Engn Lab Vasc Implants, Minist Educ,Bioengn Coll, Chongqing 400030, Peoples R China; [Wang, Guixue; Zang, Guangchao; Zhang, Yuchan] Jinfeng Lab, Chongqing 401329, Peoples R China; [Wang, Guixue] Chongqing Med Univ, Dept Pathophysiol, Chongqing 400016, Peoples R China; [Hu, Jianjun] Guizhou Prov Peoples Hosp, Dept Pathol, Guiyang 550002, Guizhou, Peoples R China"

通信作者："Zang, G; Zhang, Y (通讯作者)，Chongqing Med Univ, Inst Life Sci & Lab Tissue & Cell Biol, Lab Teaching Management Ctr, Chongqing 400016, Peoples R China.; Zang, G; Zhang, Y (通讯作者)，Jinfeng Lab, Chongqing 401329, Peoples R China.; Wang, G (通讯作者)，Chongqing Med Univ, Dept Pathophysiol, Chongqing 400016, Peoples R China."

来源：RESEARCH

ESI学科分类：　

WOS号：WOS:000993638100001

JCR分区：Q1

影响因子：8.5

年份：2023

卷号：6

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："Multiple signal strategies remarkably improve the accuracy and efficiency of electrochemiluminescence (ECL) immunoassays, but the lack of potential-resolved luminophore pairs and chemical cross talk hinders their development. In this study, we synthesized a series of gold nanoparticles (AuNPs)/reduced graphene oxide (Au/rGO) composites as adjustable oxygen reduction reaction and oxygen evolution reaction catalysts to promote and modulate tris(2,2 ' -bipyridine) ruthenium(II) (Ru(bpy)32+)'s multisignal luminescence. With the increase in the diameter of AuNPs (3 to 30 nm), their ability to promote Ru(bpy)32+'s anodic ECL was first impaired and then strengthened, and cathodic ECL was first enhanced and then weakened. Au/ rGOs with medium-small and medium-large AuNP diameters remarkably increased Ru(bpy)32+'s cathodic and anodic luminescence, respectively. Notably, the stimulation effects of Au/rGOs were superior to those of most existing Ru(bpy)32+ co-reactants. Moreover, we proposed a novel ratiometric immunosensor construction strategy using Ru(bpy)32+'s luminescence promoter rather than luminophores as tags of antibodies to achieve signal resolution. This method avoids signal cross talk between luminophores and their respective co-reactants, which achieved a good linear range of 10-7to 10-1 ng/ml and a limit of detection of 0.33 fg/ml for detecting carcinoembryonic antigen. This study addresses the previous scarcity of the macromolecular co-reactants of Ru(bpy)32+, broadening its application in biomaterial detection. Furthermore, the systematic clarification of the detailed mechanisms for converting the potential-resolved luminescence of Ru(bpy)32+ could facilitate an in-depth understanding of the ECL process and should inspire new designs of Ru(bpy)32+ luminescence enhancers or applications of Au/ rGOs to other luminophores. This work removes some impediments to the development of multisignal ECL biodetection systems and provides vitality into their widespread applications."

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