Comprehensive analysis and identification of prognostic biomarkers and immunotherapeutic targets in the NADPH oxidase family (and its regulatory subunits) in pancreatic ductal adenocarcinoma

作者全名："Guo, Ruiqi; Zheng, Panchun; Zhu, Shasha; Zeng, Zhen; Li, Zhenyu; Yang, Yaying"

作者地址："[Guo, Ruiqi; Zheng, Panchun; Yang, Yaying] Chongqing Med Univ, Coll Basic Med, Dept Pathol, Chongqing, Peoples R China; [Guo, Ruiqi; Zheng, Panchun; Yang, Yaying] Chongqing Med Univ, Mol Med Diagnost & Testing Ctr, Chongqing, Peoples R China; [Guo, Ruiqi; Zheng, Panchun; Yang, Yaying] Chongqing Med Univ, Affiliated Hosp 1, Dept Pathol, Chongqing, Peoples R China; [Zhu, Shasha] Chongqing Med Univ, Affiliated Hosp 1, Ctr Clin Mol Med Detect, Chongqing, Peoples R China; [Zeng, Zhen; Li, Zhenyu] Chongqing Univ, Dept Pathol, Canc Hosp, Chongqing, Peoples R China"

通信作者："Yang, YY (通讯作者)，Chongqing Med Univ, Coll Basic Med, Dept Pathol, Chongqing, Peoples R China.; Yang, YY (通讯作者)，Chongqing Med Univ, Mol Med Diagnost & Testing Ctr, Chongqing, Peoples R China.; Yang, YY (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Pathol, Chongqing, Peoples R China.; Li, ZY (通讯作者)，Chongqing Univ, Dept Pathol, Canc Hosp, Chongqing, Peoples R China."

来源：CLINICAL & TRANSLATIONAL ONCOLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000994112700002

JCR分区：Q2

影响因子：2.8

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：NADPH oxidase family; Pancreatic ductal adenocarcinoma; NK cells; Immunotherapy; Tumor immune microenvironment

摘要："PurposeThis study aimed to evaluate the role of NADPH in pancreatic ductal adenocarcinoma using bioinformatic analyses and experimental validations.MethodsWe compared the expression levels, performed GO and KEGG analysis of NADPH oxidase family and its regulatory subunits, and determined the survival of patients with pancreatic ductal adenocarcinoma by GEPIA, David and KM plotter. The relationship between their expression with immune infiltration levels, phagocytotic/NK cell immune checkpoints, recruitment-related molecules were detected by Timer 2.0 and TISIDB, respectively. Subsequently, their correlation with NK cell infiltration level was verified by immunohistochemistry.ResultsThe expression of some members of the NADPH oxidase family and its regulatory subunits was significantly increased in pancreatic ductal adenocarcinoma tissues compared to that in normal tissues and was positively correlated with natural killer (NK) cell infiltration. Furthermore, the NADPH oxidase family and its regulatory subunits were associated with survival and immune status in patients with pancreatic ductal adenocarcinoma, including chemokines, immune checkpoints, and immune infiltration levels of NK cells, monocytes, and myeloid-derived suppressor cells.ConclusionsThese results suggest the NADPH oxidase family and its regulatory subunits might serve as indicators for predicting the responsiveness to immunotherapy and outcome of patients with pancreatic ductal adenocarcinoma, providing a new perspective or strategy for immunotherapy in pancreatic ductal adenocarcinoma."

基金机构："Program for Youth Innovation in Future Medicine, Chongqing Medical University [W0096]; Chongqing Medical University"

基金资助正文："This research was funded by Program for Youth Innovation in Future Medicine, Chongqing Medical University, grant number W0096 and the APC was funded by Chongqing Medical University."