Empagliflozin improves kidney senescence induced by d-galactose by reducing sirt1-mediated oxidative stress

作者全名："Fang, Ronghua; Chen, Jie; Long, Jiangchuan; Zhang, Binghan; Huang, Qixuan; Li, Shengbing; Li, Ke; Chen, Qing; Liu, Dongfang"

作者地址："[Fang, Ronghua; Chen, Jie; Long, Jiangchuan; Zhang, Binghan; Huang, Qixuan; Li, Shengbing; Li, Ke; Chen, Qing; Liu, Dongfang] Chongqing Med Univ, Dept Endocrinol, Affiliated Hosp 2, Chongqing 400010, Peoples R China; [Chen, Jie] Ninth Peoples Hosp Chongqing, Dept Endocrinol, Chongqing 400700, Peoples R China; [Zhang, Binghan] Chongqing Gen Hosp, Dept Endocrinol, Chongqing 401147, Peoples R China"

通信作者："Liu, DF (通讯作者)，Chongqing Med Univ, Dept Endocrinol, Affiliated Hosp 2, Chongqing 400010, Peoples R China."

来源：BIOGERONTOLOGY

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000994724900001

JCR分区：Q1

影响因子：4.4

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：Empagliflozin; d-galactose; Ageing; Kidney; Sirt1; Redox signalling

摘要："Sodium-glucose cotransporter-2 (SGLT-2) inhibitors have received widespread attention because of their significant protective effects on the kidney. Previous studies have shown that Sirt1, as which is an antiaging protein, is closely related to the maintenance of redox homeostasis. The goal of this study was to determine whether empagliflozin could ameliorate d-galactose-induced renal senescence in mice, and examine the possible mechanisms of Sirt1. We constructed a rapid ageing model in mice by administering d-galactose. An ageing model was constructed by treating cells with high glucose. Treadmill and Y-maze tests were used to assess exercise tolerance and learning memory ability. Pathologically stained sections were used to assess kidney injury. Tissue and cell senescence were evaluated by senescence-associated beta-galactosidase staining. The expression levels of P16, SOD1, SOD2 and Sirt1 were detected by immunoblotting. d-gal-treated mice exhibited significant age-related changes, as measured by behavioural tests and ageing marker protein levels. empagliflozin alleviated these ageing manifestations. In addition, Sirt1, SOD1 and SOD2 levels were downregulated in model mice and upregulated by empagliflozin treatment. Empagliflozin had similar protective effects at the cellular level, and these effects were reduced by the Sirt1 inhibitor. Empagliflozin has an antiaging effect, which may be related to reducing Sirt1-mediated oxidative stress."

基金机构：research fund from Chongqing Municipal Health Commission [CQYC2020020331]; Chongqing Municipal Bureau of Science and Technology [cstc2021ycjh-bgzxm0050]

基金资助正文："This work was supported by the research fund from Chongqing Municipal Health Commission (Grant No. CQYC2020020331), and Chongqing Municipal Bureau of Science and Technology (Grant No. cstc2021ycjh-bgzxm0050)."