Zinc-finger protein 382 antagonises CDC25A and ZEB1 signaling pathway in breast cancer

作者全名："Li, Shuman; He, Xiaoqian; Wang, Yan; Chen, Weihong; Sun, Ran; Tian, Shaorong; He, Sanxiu; Pu, Chunyun; Li, Chen; Zhou, Dishu; Jiang, Yu; Tao, Qian; Li, Lili; Ye, Lin; Wu, Yue; Peng, Weiyan; Xiang, Tingxiu"

作者地址："[Li, Shuman] Chongqing Med Univ, Affiliated Hosp 2, Dept Oncol, Chongqing 400016, Peoples R China; [Li, Shuman; Xiang, Tingxiu] Chongqing Univ Canc Hosp, Chongqing Key Lab Translat Res Canc Metastasis & I, Chongqing 400030, Peoples R China; [He, Xiaoqian; Wang, Yan; Chen, Weihong; Sun, Ran; Tian, Shaorong; He, Sanxiu; Pu, Chunyun; Zhou, Dishu; Jiang, Yu; Ye, Lin; Wu, Yue; Peng, Weiyan; Xiang, Tingxiu] Chongqing Med Univ, Affiliated Hosp 1, Key Lab Mol Oncol & Epigenet, Chongqing 400016, Peoples R China; [Li, Chen; Tao, Qian; Li, Lili] Chinese Univ Hong Kong, Li Ka Shing Inst Hlth Sci, Sir YK Pao Ctr Canc, Canc Epigenet Lab,Dept Clin Oncol,State Key Lab Tr, Shenzhen 518172, Guangdong, Peoples R China; [Li, Chen; Tao, Qian; Li, Lili] CUHK Shenzhen Res Inst, Shenzhen 518172, Guangdong, Peoples R China; [Xiang, Tingxiu] Chongqing Univ, Canc Hosp, Chongqing Key Lab Translat Res Canc Metastasis & I, Chongqing 400030, Peoples R China"

通信作者："Xiang, TX (通讯作者)，Chongqing Univ, Canc Hosp, Chongqing Key Lab Translat Res Canc Metastasis & I, Chongqing 400030, Peoples R China."

来源：GENES & DISEASES

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000995851500001

JCR分区：Q1

影响因子：6.8

年份：2023

卷号：10

期号：2

开始页：568

结束页：582

文献类型：Article

关键词：Breast cancer; CDC25A; EMT; ZEB1; ZNF382

摘要："Our previous studies found that Zinc-finger protein 382 (ZNF382) played as a tumor suppressor gene in esophageal and gastric cancers, and a positive correlation between the high expression of ZNF382 and better outcome in breast cancer patients. However, the biological roles and mechanisms of ZNF382 in breast cancer remains unclear. We detected ZNF382 expression by reverse-transcription PCR (RT-PCR) and real-time quantitative PCR (qRT-PCR) in breast cancer cells and tissues, and explored the impacts and mechanisms of ectopic ZNF382 expression in breast cancer cells in vitro and in vivo, respectively. Our results revealed that ZNF382 was significantly down-regulated in breast cancer tissues compared with adjacent non-cancer tissues. Restoration of ZNF382 expression in silenced breast cancer cells not only inhibited tumor cell colony formation, viability, migration and invasion, and epithelial mesenchymal-transition (EMT), but also induced apoptosis and G0/G1 arrest. In conclusion, ZNF382 could induce G0/G1 cell cycle arrest through inhibiting CDC25A signaling, and, inhibit cell migration, invasion and EMT by antagonizing ZEB1 signaling in breast cancer cells. (c) 2022 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons."

基金机构："National Natural Science Foundation of China [81872380, 81772869]; Natural Science Foundation of Chongqing [2019ZX002, cstc2019jcjy-msxmX0861, cstc2020jcyj-bshX0025]; Opening Foundation of Chongqing Key Laboratory of Mo- lecular Oncology and Epigenetics [MOEL201702]; Post- doctoral Science Fundation of China [2020M683262]; National Key Research and Development Program of China [2017YFE01 91700]; HK RGC [GRF14115019]"

基金资助正文："This study was supported by National Natural Science Foundation of China (No. 81872380, 81772869) , Natural Science Foundation of Chongqing (No. 2019ZX002, cstc2019jcjy-msxmX0861, cstc2020jcyj-bshX0025) and Opening Foundation of Chongqing Key Laboratory of Molecular Oncology and Epigenetics (No. MOEL201702) , Post- doctoral Science Fundation of China (No. 2020M683262) , National Key Research and Development Program of China (No. 2017YFE01 91700) and HK RGC (No. GRF14115019) ."