A robust high selectivity fluorescence turn-on nanoprobe for peroxynitrite detection in inflammatory cells and mice

作者全名："Ou, Pinghua; Ran, Hongyan; Ye, Xiaoping; Wang, Junyi; Pang, Meiling; Zhao, Lulu; Chen, Meizi; Li, Xiong; Ma, Yongping; Wang, Ping; Chen, Jin; Luo, Quan; Peng, Yongbo"

作者地址："[Ou, Pinghua] Cent South Univ, Xiangya Hosp 3, Dept Stomatol, Changsha 410013, Peoples R China; [Ran, Hongyan; Wang, Junyi; Pang, Meiling; Zhao, Lulu; Ma, Yongping; Peng, Yongbo] Chongqing Med Univ, Coll Pharm, Chongqing Key Lab Pharmaceut Metab Res, Key Lab Biochem & Mol Pharmacol, Chongqing 400016, Peoples R China; [Ye, Xiaoping] Southern Med Univ, Nanfang Hosp, Baiyun Branch, Guangzhou 510515, Peoples R China; [Chen, Meizi] First Peoples Hosp Chenzhou, Dept Resp Med, Chenzhou 423000, Peoples R China; [Li, Xiong] Guangdong Pharmaceut Univ, Sch Clin Pharm, Guangzhou 510006, Peoples R China; [Li, Xiong] Guangdong Pharmaceut Univ, Affiliated Hosp 1, Guangzhou 510006, Peoples R China; [Wang, Ping; Chen, Jin] Chongqing Med Univ, Affiliated Hosp 1, Dept Dermatol, 1 Youyi Rd, Chongqing 400016, Peoples R China; [Luo, Quan] Hunan Normal Univ, Affiliated Hosp 1, Hunan Prov Peoples Hosp, Dept Rehabil, Changsha 410000, Peoples R China"

通信作者："Peng, YB (通讯作者)，Chongqing Med Univ, Coll Pharm, Chongqing Key Lab Pharmaceut Metab Res, Key Lab Biochem & Mol Pharmacol, Chongqing 400016, Peoples R China.; Luo, Q (通讯作者)，Hunan Normal Univ, Affiliated Hosp 1, Hunan Prov Peoples Hosp, Dept Rehabil, Changsha 410000, Peoples R China."

来源：SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY

ESI学科分类：CHEMISTRY

WOS号：WOS:000996056600001

JCR分区：Q1

影响因子：4.3

年份：2023

卷号：291

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Peroxynitrite anion (ONOO; Inflammation; Inflammatory diseases; Fluorescent nanoprobe

摘要："Changed levels of intracellular peroxynitrite anion (ONOO-) are closely related to the occurrence and devel-opment of inflammation. Specific imaging of ONOO- at sites of inflammation can be of great significance not only for inflammation diagnosis but also for obtaining a deeper understanding of the role of ONOO- in inflammation. Therefore, there is an urgent need for constructing some reliable tools to study the relationship between ONOO- and inflammation in biosystems. In this work, we developed a robust high selectivity fluo-rescence turn-on nanoprobe (Rhb-ONOO) for inflammation-targeted imaging of ONOO-. The Rhb-ONOO was obtained by self-assembly of amphiphilic Rhb-ONOO, which was constructed by the condensation reaction of the hydrophobic, ONOO--response and deep red-emitting fluorophore (Rhb) with hydrophilic biopolymer glycol chitosan (GC). Rhb-ONOO showed rapid response towards ONOO- during 60 s, high sensitivity with 19-fold enhancement of fluorescence intensity ratio (I628/I0), and excellent selectivity towards ONOO- over other analytes as well as a good linear relationship was observed between the I628/I0 and the ONOO- concentration range 0-1 mu M, with an excellent limit of detection (LOD) of 33 nM. Impressively, it was successfully employed Rhb-ONOO for ONOO- imaging in living inflammatory cells and drug-induced inflammatory mice, illustrating nanoprobe Rhb-ONOO has excellent potential for further study ONOO--related inflammatory diseases."

基金机构：Natural Science Foundation of Hunan Province [2022JJ70002]; Future Medical Youth Innova- tion Team of Chongqing Medical University [2022-W0032-PI/Jin Chen]

基金资助正文：This work was partially supported by Natural Science Foundation of Hunan Province (2022JJ70002) and the Future Medical Youth Innova- tion Team of Chongqing Medical University (2022-W0032-PI/Jin Chen and Yongbo Peng) .