An improved method for preparing stained ground teeth sections

作者全名："Qin, Qizhong; Li, Yueheng; Zhou, Yujian"

作者地址："[Qin, Qizhong; Zhou, Yujian] Chongqing Med Univ, Expt Teaching & Management Ctr, Chongqing, Peoples R China; [Li, Yueheng] Chongqing Med Univ, Stomatol Hosp, Chongqing, Peoples R China; [Li, Yueheng] Chongqing Med Univ, Coll Stomatol, Chongqing, Peoples R China; [Li, Yueheng] Chongqing Med Univ, Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China; [Li, Yueheng] Chongqing Med Univ, Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing, Peoples R China"

通信作者："Zhou, YJ (通讯作者)，Chongqing Med Univ, Expt Teaching & Management Ctr, Chongqing, Peoples R China."

来源：PEERJ

ESI学科分类：Multidisciplinary

WOS号：WOS:000996334200002

JCR分区：Q2

影响因子：2.3

年份：2023

卷号：11

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Ground sections of teeth; Rosin; Un-demineralization

摘要："Objective. In oral histopathology teaching and research, there is a need for high-quality undemineralized tooth sections that are easy to handle, have controlled thickness, allow the observation of intact microstructures, and can be preserved for long periods of time. Methods. Teeth were collected under non-demineralizing conditions. Tooth sections (15-25 mu m) were prepared using a diamond knife, then randomly divided into three groups: (1) stained with rosin, (2) stained with hematoxylin and eosin, or (3) not stained. The prepared tooth sections were evaluated by microscopy for clarity and microstructure visibility. Results. The use of a diamond knife in the sectioning and grinding process yielded high-quality ground sections of teeth. Rosin-stained ground sections allowed better identification of microstructures within the teeth, compared with unstained or hematoxylin and eosin-stained ground sections. Conclusion. The best results were obtained in the ground sections of teeth that were stained with rosin. Ground sections of teeth prepared using this staining method could be useful in oral histopathology teaching and research."

基金机构："Education Commission of Chongqing Municipality [KJ1500211]; First Batch of Key Disciplines On Public Health in Chongqing; College of Stomatology, Chongqing Medical University [KQJ202205]"

基金资助正文："This study was supported by the Education Commission of Chongqing Municipality (KJ1500211) and the First Batch of Key Disciplines On Public Health in Chongqing; College of Stomatology, Chongqing Medical University (KQJ202205) . The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript."