Effect of a protein kinase B (Akt) inhibitor on the angiogenesis of HUVECs and corneal neovascularization

作者全名："Wang, Xing; Wang, Peng"

作者地址："[Wang, Xing; Wang, Peng] Chongqing Med Univ, Chongqing Key Lab Ophthalmol, Affiliated Hosp 1, 1 Youyi Rd, Chongqing 400016, Peoples R China; [Wang, Xing; Wang, Peng] Chongqing Med Univ, Chongqing Eye Inst, Affiliated Hosp 1, 1 Youyi Rd, Chongqing 400016, Peoples R China"

通信作者："Wang, P (通讯作者)，Chongqing Med Univ, Chongqing Key Lab Ophthalmol, Affiliated Hosp 1, 1 Youyi Rd, Chongqing 400016, Peoples R China.; Wang, P (通讯作者)，Chongqing Med Univ, Chongqing Eye Inst, Affiliated Hosp 1, 1 Youyi Rd, Chongqing 400016, Peoples R China."

来源：WIENER KLINISCHE WOCHENSCHRIFT

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000998539200001

JCR分区：Q2

影响因子：1.9

年份：2023

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期号：　

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结束页：　

文献类型：Article; Early Access

关键词：Akt inhibitor; Angiogenesis; Corneal neovascularization; HUVECs; VEGF

摘要："BackgroundCorneal neovascularization (CNV) is a vision-threatening disease and an increasing public health concern. It was found that administering an Akt inhibitor in the second phase of retinopathy significantly decreased retinal neovascularization.MethodsThis study investigated the effect of an Akt inhibitor on the angiogenesis of human umbilical vein endothelial cells (HUVECs) and its impacts on the degree of CNV and corneal opacity in a rat keratoplasty model. Cell Counting Kit-8 (CCK-8) and 5-ethynyl-2 '-deoxyuridine (EdU) assays, tube formation assays, cell scratch experiments, and a fully allogeneic corneal transplant model were performed.ResultsIt was found that an Akt inhibitor inhibited the proliferation, angiogenesis, and migration of HUVECs induced by vascular endothelial growth factor (VEGF). The results showed that both CNV and corneal opacity were decreased in rats after Akt inhibitor administration.ConclusionThe research illustrates the vital role of Akt inhibitors in mediating CNV. The analysis shows that the Akt inhibitor may provide a novel and feasible therapeutic approach to prevent CNV, but its mechanism needs further investigation."

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