Inhibition of miR-20a by pterostilbene facilitates prostate cancer cells killed by NK cells via up-regulation of NKG2D ligands and TGF-beta 1down-regulation

作者全名："Youlin, Kuang; Simin, Liang; Jian, Kang; Li, Zhang"

作者地址："[Youlin, Kuang; Simin, Liang; Jian, Kang] Chongqing Med Univ, Affiliated Hosp 1, Dept Urol, Chongqing 400016, Peoples R China; [Li, Zhang] Chongqing Med Univ, Dept Gastroenterol, Affiliated Hosp 1, Chongqing 400016, Peoples R China"

通信作者："Li, Z (通讯作者)，Chongqing Med Univ, Dept Gastroenterol, Affiliated Hosp 1, Chongqing 400016, Peoples R China."

来源：HELIYON

ESI学科分类：　

WOS号：WOS:000998604100001

JCR分区：Q1

影响因子：3.4

年份：2023

卷号：9

期号：4

开始页：　

结束页：　

文献类型：Article

关键词：Prostate cancer; miR-20a; MICA/B; NKG2D; Natural killer cell; TGF-beta 1; Pterostilbene

摘要："Natural killer (NK) cells play a potent role in antitumor immunity via spontaneously eliminating tumor directly. However, some tumors such as prostate cancer constantly escape this immune response by down-regulating cell surface molecule recognition and/or secreting immune impressive cytokines. Here, we found pterostilbene, a natural agent with potent anticancer activity, could enhance expression of major histocompatibility complex class I chain-related proteins A and B (MICA/B) on prostate cancer cells surface, which are ligands of the natural killer group 2 member D (NKG2D) expressed by NK cells, and inhibit TGF-beta 1 secretion by prostate cancer cells. Further, we discovered that these effects were caused by inhibition of miR-20a in prostate cancer cells by pterostilbene. MiR-20a could target the 3' untranslated region (UTR) of MICA/B, resulting in their expression down-regulation. Inhibition of TGF-beta 1 function by its specific antibody attenuated its impairment to NKG2D on NK cells. Finally, we observed that pterostilbene-treated prostate cancer cells were more easily to be killed by NK cells. Taken together, our findings demonstrated inhibition of miR-20a by pterostilbene in prostate cancer cells could increase MICA/B expression and decrease TGF-beta 1 secretion, which enhanced NK cell-mediated cytotoxicity againt prostate cancer cells, suggesting a potential approach for increasing anti-prostate cancer immune."

基金机构："Project of Chongqing Science and Technology Commission [cstc2020jcyj-msxmX0085]; High-level Medical Reserved Personnel Training Project of Chongqing, Chongqing Medical Scientific Research Project [2023GDRC013]"

基金资助正文："Project of Chongqing Science and Technology Commission (No. cstc2020jcyj-msxmX0085) , High-level Medical Reserved Personnel Training Project of Chongqing, Chongqing Medical Scientific Research Project (No. 2023GDRC013) ."