PdPtRu trimetallic nanozymes and application to electrochemical immunosensor for sensitive SARS-COV-2 antigen detection

作者全名："Li, Ying; Xue, Jian; Yu, Rongjun; Chen, Siling; Deng, Xiangyu; Chen, Anyi; Qiu, Jingfu"

作者地址："[Li, Ying; Xue, Jian; Chen, Siling; Chen, Anyi; Qiu, Jingfu] Chongqing Med Univ, Sch Publ Hlth, Chongqing 400016, Peoples R China; [Xue, Jian] Zunyi Med & Pharmaceut Coll, Hlth Management Dept, Zunyi 563006, Guizhou, Peoples R China; [Yu, Rongjun; Deng, Xiangyu] Chongqing Med Univ, Univ Town Hosp, Dept Clin Lab, Chongqing 401331, Peoples R China"

通信作者："Chen, AY; Qiu, JF (通讯作者)，Chongqing Med Univ, Sch Publ Hlth, Chongqing 400016, Peoples R China."

来源：TALANTA

ESI学科分类：CHEMISTRY

WOS号：WOS:000998633400001

JCR分区：Q1

影响因子：5.6

年份：2023

卷号：260

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Trimetallic nanozyme; Electro-catalytic activity; Electrochemical immunosensor; SARS-COV-2; Antigen detection

摘要："Herein, a ternary PdPtRu nanodendrite as novel trimetallic nanozyme was reported, which possessed excellent peroxidase-like activity as well as electro-catalytic activity on account of the synergistic effect between the three metals. Based on the excellent electro-catalytic activity of trimetallic PdPtRu nanozyme toward the reduction of H2O2, the trimetallic nanozyme was applied to construct a brief electrochemical immunosensor for SARS-COV-2 antigen detection. Concretely, trimetallic PdPtRu nanodendrite was used to modify electrode surface, which not only generated high reduction current of H2O2 for signal amplification, but also provided massive active sites for capture antibody (Ab1) immobilization to construct immunosensor. In the presence of target SARS-COV-2 an-tigen, SiO2 nanosphere labeled detection antibody (Ab2) composites were introduced on the electrode surface according sandwich immuno-reaction. Due to the inhibitory effect of SiO2 nanosphere on the current signal, the current signal was decreased with the increasing target SARS-COV-2 antigen concentration. As a result, the proposed electrochemical immunosensor presented sensitive detection of SARS-COV-2 antigen with linear range from 1.0 pg/mL to 1.0 mu g/mL and limit of detection down to 51.74 fg/mL. The proposed immunosensor provide a brief but sensitive antigen detection tool for rapid diagnosis of COVID-19."

基金机构："National Natural Science Foundation of China [22204014]; Natural Science Foundation of Chongqing [cstc2020jcyj-zdxm0015, cstb2022nscq-bhx0008]; China Postdoctoral Science Foundation [2020M683259]; Science and Technology Sup- port Program of Zunyi Science and Technology Bureau [NS (2020) 22]"

基金资助正文："This work was sponsored by National Natural Science Foundation of China (22204014) , Natural Science Foundation of Chongqing (cstc2020jcyj-zdxm0015, cstb2022nscq-bhx0008) , China Postdoctoral Science Foundation (2020M683259) , the Science and Technology Sup- port Program of Zunyi Science and Technology Bureau [NS (2020) 22] ."