An engineered dual-functional peptide with high affinity to demineralized dentin enhanced remineralization efficacy in vitro and in vivo

作者全名："Li, Meng; Tu, Yuanyuan; Zhu, Wanchun; Fan, Menglin; Zhou, Zilin; Yu, Zhaohan; Wang, Shunhua; Yang, Yingming; Li, Jiyao; Liang, Kunneng"

作者地址："[Li, Meng; Tu, Yuanyuan; Fan, Menglin; Zhou, Zilin; Yu, Zhaohan; Wang, Shunhua; Yang, Yingming; Li, Jiyao; Liang, Kunneng] Sichuan Univ, West China Hosp Stomatol, Natl Clin Res Ctr Oral Dis, Dept Cariol & Endodont,State Key Lab Oral Dis, Chengdu 610041, Peoples R China; [Zhu, Wanchun] Chongqing Med Univ, Coll Stomatol, Chongqing 401147, Peoples R China"

通信作者："Liang, KN (通讯作者)，Sichuan Univ, West China Hosp Stomatol, Natl Clin Res Ctr Oral Dis, Dept Cariol & Endodont,State Key Lab Oral Dis, Chengdu 610041, Peoples R China."

来源：JOURNAL OF MATERIALS CHEMISTRY B

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:000999002400001

JCR分区：Q1

影响因子：6.1

年份：2023

卷号：11

期号：23

开始页：5170

结束页：5184

文献类型：Article

关键词：　

摘要："Dental caries continues to be a major global public health problem. Remineralization of demineralized dentin is regarded as one of the hotspots in the current study in the treatment of dental caries. However, traditional remineralization agents, which usually lack the ability to bind to demineralized dentin collagen, are easily removed by the fluids in the oral cavity, thus decreasing the remineralization efficacy. Non-collagenous proteins (NCPs) have significant effects on the biomineralization of dentin due to their dual high binding capacity to the collagen fibers and minerals. But NCPs are hard to extract, store and use directly. Inspired by the biological behavior of NCPs, in this study, we selected two functional sequences of NCPs to develop a novel and engineered dual-functional peptide (which is referred to as CYP) with collagen-binding and mineral-absorbing capability. The binding ability of CYP to collagen fibers and demineralized dentin was investigated, and the results suggested that CYP was endowed with good binding capacity to demineralized dentin, which could resist the washing of the fluid. In addition, we confirmed that CYP exerted formidable remineralization effects in collagen fibers and demineralized dentin following an in vitro remineralization regimen. Furthermore, the dual functions of CYP with good biocompatibility can simultaneously bind collagen and induce nanocrystal precipitation, thereby significantly absorbing calcium and phosphorus ions to form regenerated minerals for reversing the tooth decay process in the rat caries model. Overall, the dual functional peptide CYP fabricated in this study provides an ideal and smart strategy for dentin remineralization and the treatment of caries."

基金机构："National Natural Science Foundation of China [82270970, 82170949, 81991500, 81991501]; Key Research and Development Program of Sichuan Province [2022YFS0288, 2023YFS0048]; Chengdu Science and Technology Support Program [2021-YF05-02061-SN]; Sichuan University [0040304153013]; China Postdoctoral Foundation [2018M643507]"

基金资助正文："This work was supported by the National Natural Science Foundation of China (82270970, 82170949, 81991500 and 81991501), the Key Research and Development Program of Sichuan Province (2022YFS0288 and 2023YFS0048), the Chengdu Science and Technology Support Program (2021-YF05-02061-SN), the Postdoctoral Cross Funding of Sichuan University (0040304153013), and the China Postdoctoral Foundation 2018M643507 (L. K. N)."