Vitamin D receptor (VDR) on the cell membrane of mouse macrophages participates in the formation of lipopolysaccharide tolerance: mVDR is related to the effect of artesunate to reverse LPS tolerance

作者全名："Zhang, Yu; Zhou, Jun; Hua, Ling; Li, Pan; Wu, Jiaqi; Shang, Shenglan; Deng, Fei; Luo, Jing; Liao, Mengling; Wang, Nuoyan; Pan, Xichun; Yuan, Yue; Zheng, Yue; Lu, Yonglin; Huang, Yasi; Zheng, Jiang; Liu, Xin; Li, Xiaoli; Zhou, Hong"

作者地址："[Zhang, Yu; Zhou, Jun; Hua, Ling; Deng, Fei; Luo, Jing; Liao, Mengling; Wang, Nuoyan; Yuan, Yue; Huang, Yasi; Zhou, Hong] Zunyi Med Univ, Key Lab Basic Pharmacol, Minist Educ, Zunyi 563000, Guizhou, Peoples R China; [Zhang, Yu; Zhou, Jun; Hua, Ling; Deng, Fei; Luo, Jing; Liao, Mengling; Wang, Nuoyan; Yuan, Yue; Huang, Yasi; Zhou, Hong] Zunyi Med Univ, Joint Int Res Lab Ethnomed, Minist Educ, Zunyi 563000, Guizhou, Peoples R China; [Li, Pan; Pan, Xichun] Army Med Univ, Mil Med Univ 3, Coll Pharm, Dept Pharmacol, Chongqing 400038, Peoples R China; [Wu, Jiaqi; Shang, Shenglan; Zheng, Yue; Lu, Yonglin; Zheng, Jiang; Liu, Xin] Army Med Univ, Mil Med Univ 3, Southwest Hosp, Med Res Ctr, Chongqing 400038, Peoples R China; [Li, Xiaoli] Chongqing Med Univ, Coll Pharm, Dept Pharmacol, Chongqing 400016, Peoples R China; [Li, Xiaoli] Chongqing Key Lab Drug Metab, Chongqing 400016, Peoples R China"

通信作者："Zhou, H (通讯作者)，Zunyi Med Univ, Key Lab Basic Pharmacol, Minist Educ, Zunyi 563000, Guizhou, Peoples R China.; Zhou, H (通讯作者)，Zunyi Med Univ, Joint Int Res Lab Ethnomed, Minist Educ, Zunyi 563000, Guizhou, Peoples R China.; Liu, X (通讯作者)，Army Med Univ, Mil Med Univ 3, Southwest Hosp, Med Res Ctr, Chongqing 400038, Peoples R China.; Li, XL (通讯作者)，Chongqing Med Univ, Coll Pharm, Dept Pharmacol, Chongqing 400016, Peoples R China.; Li, XL (通讯作者)，Chongqing Key Lab Drug Metab, Chongqing 400016, Peoples R China."

来源：CELL COMMUNICATION AND SIGNALING

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000999021500001

JCR分区：Q1

影响因子：8.2

年份：2023

卷号：21

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Membrane vitamin D receptor; LPS tolerance; Artesunate; Lipid raft; Caveolin-1

摘要："It is unclear whether membrane vitamin D receptor (mVDR) exists on the macrophage membrane or whether mVDR is associated with lipopolysaccharide (LPS) tolerance. Herein, we report that interfering with caveolae and caveolae-dependent lipid rafts inhibited the formation of LPS tolerance. VDR was detected as co-localized with membrane molecular markers. VDR was detected on the cell membrane and its level was higher in LPS-tolerant cells than that in only LPS treatment cells. Anti-VDR antibodies could abolish the effect of artesunate (AS) to reverse LPS tolerance, and the wild-type peptides (H397 and H305) of VDR, but not the mutant peptide (H397D and H305A), led to the loss of AS's effect. AS decreased the mVDR level in LPS-tolerant cells. In vivo, AS significantly reduced VDR level in the lung tissue of LPS-tolerant mice. In summary, mVDR exists on the cell membrane of macrophages and is closely associated with the formation of LPS tolerance and the effects of AS."

基金机构："National Natural Science Foundation of China [81872914]; National Natural Science Foundation of China-Guizhou Provincial People's Government Joint Fund Project [NSFC-U1812403-41]; Major National Science and Technology Program of China for Innovative Drug [2017ZX09101002-002009]; Fourth Batch of ""Thousand People Innovation and Entrepreneurship Talents Fund"" in Guizhou Province, China"

基金资助正文："This work was supported by grants from the National Natural Science Foundation of China (81872914), the National Natural Science Foundation of China-Guizhou Provincial People's Government Joint Fund Project, sub-project (grant number NSFC-U1812403-4-1), the Major National Science and Technology Program of China for Innovative Drug (2017ZX09101002-002-009), and the Fourth Batch of ""Thousand People Innovation and Entrepreneurship Talents Fund"" in Guizhou Province, China."