miR-138-5p targets MCU to inhibit mitochondrial biogenesis and colorectal cancer growth

作者全名："Zhu, Jianjun; Zhang, Chunle; Wang, Zhengjie; Shi, Lihong; Li, Li; Wu, Hao; Liu, Ming"

作者地址："[Zhu, Jianjun; Li, Li; Wu, Hao; Liu, Ming] Shanxi Med Univ, Dept Med Cellular Biol & Genet, Taiyuan, Peoples R China; [Zhang, Chunle] West China Hosp Sichuan Univ, Kidney Res Inst, Dept Nephrol, Chengdu, Peoples R China; [Wang, Zhengjie] Chongqing Med Univ, Dept Nucl Med, Affiliated Hosp 1, Chongqing, Peoples R China; [Shi, Lihong] Shanxi Med Univ, Dept Human Anat, Taiyuan, Peoples R China"

通信作者："Wu, H; Liu, M (通讯作者)，Shanxi Med Univ, Dept Med Cellular Biol & Genet, Taiyuan, Peoples R China."

来源：JOURNAL OF CELLULAR AND MOLECULAR MEDICINE

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:001000013000001

JCR分区：Q2

影响因子：5.3

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：colorectal carcinoma; MCU; miR-138-5p; ROS; tumour growth

摘要："miR-138-5p has been identified as a novel cancer-related miRNA molecule in a variety of malignancies. However, the functions and mechanisms underlying miR-138-5p in colorectal carcinoma (CRC) remains largely unknown. In the present study, we analysed the biological effects and clinical significance of miR-138-5p in CRC. miR-138-5p expression was analysed by quantitative real-time PCR in CRC tissues and cell lines. The effects of miR-138-5p on CRC cell growth was detected by cell proliferation, colony formation, cell cycle and cell apoptosis assays in vitro and in vivo. Our data showed that miR-138-5p was significantly downregulated in CRC. Downregulated miR-138-5p was related with poor prognosis in patients with CRC. miR-138-5p suppressed CRC growth but promoted cell death both in vitro and in vivo. Online predictions and integrated experiments identified that miR-138-5p targeted MCU, and downregulated miR-138-5p promoted mitochondrial biogenesis in CRC. In the light of the underlying mechanisms, our results indicated that downregulated miR-138-5p led to increased expression of MCU, which subsequently increased the production of ROS to promote CRC growth. Our results indicated that downregulated miR-138-5p strengthened mitochondrial biogenesis through targeting MCU, thus contributing to CRC cell growth, which may provide a potential therapeutic target for CRC."

基金机构："Applied Basic Research Project of Shanxi Province, China [202103021224228, 20210302124376]; National Natural Science Foundation of China [81902513]; Science Research Start -up fund for doctor of Shanxi medical university [XD1808]; Science/Technology Project of Sichuan province [2021YFS0161]"

基金资助正文："Applied Basic Research Project of Shanxi Province, China, Grant/Award Number: 202103021224228 and 20210302124376; National Natural Science Foundation of China, Grant/Award Number: 81902513; Science Research Start -up fund for doctor of Shanxi medical university, Grant/Award Number: XD1808; the Science/Technology Project of Sichuan province, Grant/Award Number: 2021YFS0161"