MicroRNA-31 induced by Fusobacterium nucleatum infection promotes colorectal cancer tumorigenesis

作者全名："Tang, Bin; Lu, Xiaoxue; Tong, Yanan; Feng, Yuyang; Mao, Yilan; Dun, Guodong; Li, Jing; Xu, Qiaolin; Tang, Jie; Zhang, Tao; Deng, Ling; He, Xiaoyi; Lan, Yuanzhi; Luo, Huaxing; Zeng, Linghai; Xiang, Yuanyuan; Li, Qian; Zeng, Dongzhu; Mao, Xuhu"

作者地址："[Tang, Bin] Chongqing Univ, Jiangjin Hosp, Sch Med, Dept Clin Lab, Chongqing 402260, Peoples R China; [Lu, Xiaoxue; Tong, Yanan; Feng, Yuyang; Dun, Guodong; Li, Qian; Mao, Xuhu] Third Mil Med Univ, Army Med Univ, Coll Pharm & Med Lab, Dept Clin Microbiol & Immunol, Chongqing 400038, Peoples R China; [Li, Jing; Xu, Qiaolin; Tang, Jie; Zhang, Tao; Deng, Ling; He, Xiaoyi; Lan, Yuanzhi; Luo, Huaxing; Zeng, Linghai; Xiang, Yuanyuan; Zeng, Dongzhu] Chongqing Med Univ, Dept Gen Surg, Affiliated Hosp 3, Chongqing 401120, Peoples R China; [Mao, Yilan] Chongqing Med Univ, Nursing Coll, Class 2021 undergrad, Chongqing 400016, Peoples R China"

通信作者："Li, Q; Mao, XH (通讯作者)，Third Mil Med Univ, Army Med Univ, Coll Pharm & Med Lab, Dept Clin Microbiol & Immunol, Chongqing 400038, Peoples R China.; Zeng, DZ (通讯作者)，Chongqing Med Univ, Dept Gen Surg, Affiliated Hosp 3, Chongqing 401120, Peoples R China."

来源：ISCIENCE

ESI学科分类：　

WOS号：WOS:001001146800001

JCR分区：Q1

影响因子：4.6

年份：2023

卷号：26

期号：5

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："Persistent Fusobacterium nucleatum infection is associated with the develop-ment of human colorectal cancer (CRC) and promotes tumorigenicity, but the underlying mechanisms remain unclear. Here, we reported that F. nucleatum promoted the tumorigenicity of CRC, which was associated with F. nucleatum- induced microRNA-31 (miR-31) expression in CRC tissues and cells. F. nucleatum infection inhibited autophagic flux by miR-31 through inhibiting syntaxin-12 (STX12) and was associated with the increased intracellular survival of F. nucleatum. Overexpression of miR-31 in CRC cells promoted their tumorige-nicity by targeting eukaryotic initiation factor 4F-binding protein 1/2 (eIF4EBP1/ 2), whereas miR-31 knockout mice were resistant to the formation of colorectal tumors. In conclusion, F. nucleatum, miR-31, and STX12 form a closed loop in the autophagy pathway, and continuous F. nucleatum-induced miR-31 expression promotes the tumorigenicity of CRC cells by targeting eIF4EBP1/2. These find-ings reveal miR-31 as a potential diagnostic biomarker and therapeutic target in CRC patients with F. nucleatum infection."

基金机构："National Natural Science Foundation of China [81501796, 82102744]; Natural Science Foundation of Chongqing [cstc2017jcyjAX0205, cstc2020jcyj-msxmX0220]; project of school management in Army Medical University [31024629]"

基金资助正文："The authors would like to thank patients with colorectal cancer who donated their specimen and time to this study. This work was supported by grants from the National Natural Science Foundation of China (no. 81501796 and 82102744) , Natural Science Foundation of Chongqing (no. cstc2017jcyjAX0205 and cstc2020jcyj-msxmX0220) , the project of school management in Army Medical University (no. 31024629) . The authors thank Medjaden Inc. for its assistance in the preparation of the manuscript."