A Novel Poly(3-hexylthiophene) Engineered Interface for Electrochemical Monitoring of Ascorbic Acid During the Occurrence of Glutamate-Induced Brain Cytotoxic Edemas

作者全名："Meng, Zexuan; Zhang, Yuchan; Yang, Lu; Zhao, Shuang; Zhou, Qiang; Chen, Jiajia; Sui, Jiuxi; Wang, Jian; Guo, Lizhong; Chang, Luyue; He, Jialing; Wang, Guixue; Zang, Guangchao"

作者地址："[Meng, Zexuan; Zhang, Yuchan; Yang, Lu; Zhou, Qiang; Chen, Jiajia; Sui, Jiuxi; Wang, Jian; Guo, Lizhong; Chang, Luyue; Zang, Guangchao] Chongqing Med Univ, Inst Life Sci, Chongqing 400016, Peoples R China; [Meng, Zexuan; Zhang, Yuchan; Yang, Lu; Zhou, Qiang; Chen, Jiajia; Sui, Jiuxi; Wang, Jian; Guo, Lizhong; Chang, Luyue; Zang, Guangchao] Chongqing Med Univ, Lab Teaching & Management Ctr, Lab Tissue & Cell Biol, Chongqing 400016, Peoples R China; [Zhao, Shuang; He, Jialing; Wang, Guixue] Chongqing Univ, Key Lab Biorheol Sci & Technol, State & Local Joint Engn Lab Vasc Implants, Minist Educ,Bioengn Coll, Chongqing 400030, Peoples R China; [Zang, Guangchao] Jinfeng Lab, Chongqing 401329, Peoples R China; [Zhou, Qiang] Chongqing Med Univ, Dept Pathophysiol, Chongqing, Peoples R China"

通信作者："Zang, GC (通讯作者)，Chongqing Med Univ, Inst Life Sci, Chongqing 400016, Peoples R China.; Zang, GC (通讯作者)，Chongqing Med Univ, Lab Teaching & Management Ctr, Lab Tissue & Cell Biol, Chongqing 400016, Peoples R China.; Wang, GX (通讯作者)，Chongqing Univ, Key Lab Biorheol Sci & Technol, State & Local Joint Engn Lab Vasc Implants, Minist Educ,Bioengn Coll, Chongqing 400030, Peoples R China.; Zang, GC (通讯作者)，Jinfeng Lab, Chongqing 401329, Peoples R China."

来源：RESEARCH

ESI学科分类：　

WOS号：WOS:001001554000001

JCR分区：Q1

影响因子：8.5

年份：2023

卷号：6

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："Although neuroelectrochemical sensing technology offers unique benefits for neuroscience research, its application is limited by substantial interference in complex brain environments while ensuring biosafety requirements. In this study, we introduced poly(3-hexylthiophene) (P3HT) and nitrogen-doped multiwalled carbon nanotubes (N-MWCNTs) to construct a composite membrane-modified carbon fiber microelectrode (CFME/P3HT-N-MWCNTs) for ascorbic acid (AA) detection. The microelectrode presented good linearity, selectivity, stability, antifouling, and biocompatibility and exhibited great performance for application in neuroelectrochemical sensing. Subsequently, we applied CFME/P3HT-N-MWCNTs to monitor AA release from in vitro nerve cells, ex vivo brain slices, and in vivo living rat brains and determined that glutamate can induce cell edema and AA release. We also found that glutamate activated the N-methyl-d-aspartic acid receptor, which enhanced Na+ and Cl- inflow to induce osmotic stress, resulting in cytotoxic edema and ultimately AA release. This study is the first to observe the process of glutamate-induced brain cytotoxic edema with AA release and to reveal the mechanism. Our work can benefit the application of P3HT in in vivo implant microelectrode construction to monitor neurochemicals, understand the molecular basis of nervous system diseases, and discover certain biomarkers of brain diseases."

基金机构："Natural Science Foundation of Chongqing [cstc2020jcyj-msxmX0330]; JinFeng Laboratory Foundation, Chongqing, China [jfkyjf202203001]; Project of Tutorial System of Medical Undergraduate in Lab Teaching and Management Center in Chongqing Medical University [LTMCMTS202109, LTMCMTS202106]; Project of Scientific Research and Innovative Experiment for College Student in Chongqing Medical University [SRIEP202011, SRIEP202047, SPIEP202167]; National Project of University Students Innovation and Entrepreneurship Training Program [S202010631016]; Chongqing Municipal Education Commission Foundation [CYS22333]"

基金资助正文："This work was supported by grants the Natural Science Foundation of Chongqing (cstc2020jcyj-msxmX0330) ; the JinFeng Laboratory Foundation, Chongqing, China (jfkyjf202203001) ; the Project of Tutorial System of Medical Undergraduate in Lab Teaching and Management Center in Chongqing Medical University (LTMCMTS202109 and LTMCMTS202106) ; the Project of Scientific Research and Innovative Experiment for College Student in Chongqing Medical University (SRIEP202011, SRIEP202047, and SPIEP202167) ; the National Project of University Students Innovation and Entrepreneurship Training Program (S202010631016) , and Chongqing Municipal Education Commission Foundation (CYS22333) ."