miR-486-5p Attenuates Steroid-Induced Adipogenesis and Osteonecrosis of the Femoral Head Via TBX2/P21 Axis

作者全名："Chen, Yu; Tang, Boyu; Jiang, Weiqian; Sun, Mingjie; Zhang, Hongrui; Tao, Yuzhang; Wang, Hongwei; Xiang, Dulei; Bai, Haobo; Guo, Mingkang; Zhao, Pei; Yan, Wenlong; Huang, Xiao; Chen, Tingmei; Lian, Chengjie; Zhang, Jian"

作者地址："[Chen, Yu; Tang, Boyu; Jiang, Weiqian; Sun, Mingjie; Zhang, Hongrui; Tao, Yuzhang; Wang, Hongwei; Xiang, Dulei; Bai, Haobo; Guo, Mingkang; Zhao, Pei; Yan, Wenlong; Huang, Xiao; Lian, Chengjie; Zhang, Jian] Chongqing Med Univ, Affiliated Hosp 1, Dept Orthoped, Chongqing, Peoples R China; [Chen, Yu; Tang, Boyu; Jiang, Weiqian; Sun, Mingjie; Zhang, Hongrui; Tao, Yuzhang; Wang, Hongwei; Xiang, Dulei; Bai, Haobo; Guo, Mingkang; Zhao, Pei; Yan, Wenlong; Huang, Xiao; Lian, Chengjie; Zhang, Jian] Chongqing Med Univ, Orthoped Lab, Chongqing, Peoples R China; [Chen, Tingmei] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing, Peoples R China; [Lian, Chengjie; Zhang, Jian] Chongqing Med Univ, Affiliated Hosp 1, Dept Orthoped, Chongqing 400000, Peoples R China"

通信作者："Lian, CJ; Zhang, J (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Orthoped, Chongqing 400000, Peoples R China."

来源：STEM CELLS

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:001002335400001

JCR分区：Q1

影响因子：4

年份：2023

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期号：　

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文献类型：Article; Early Access

关键词：steroid-induced osteonecrosis of the femoral head; mesenchymal stem cells; adipogenesis; miR-486-5p; P21; TBX2; mitotic clonal expansion

摘要："Enhanced adipogenic differentiation of mesenchymal stem cells (MSCs) is considered as a major risk factor for steroid-induced osteonecrosis of the femoral head (SOFNH). The role of microRNAs during this process has sparked interest. miR-486-5p expression was down-regulated significantly in femoral head bone tissues of both SONFH patients and rat models. The purpose of this study was to reveal the role of miR-486-5p on MSCs adipogenesis and SONFH progression. The present study showed that miR-486-5p could significantly inhibit adipogenesis of 3T3-L1 cells by suppressing mitotic clonal expansion (MCE). And upregulated expression of P21, which was caused by miR-486-5p mediated TBX2 decrease, was responsible for inhibited MCE. Further, miR-486-5p was demonstrated to effectively inhibit steroid-induced fat formation in the femoral head and prevented SONFH progression in a rat model. Considering the potent effects of miR-486-5p on attenuating adipogenesis, it seems to be a promising target for the treatment of SONFH."

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