miR-383-5p serves as a tumor suppressor in bladder cancer by suppressing PI3K/AKT signaling pathway

作者全名："Liu, Yu; Yu, Haitao; Zeng, Bin; Gou, Xin; Ren, Ke; Yuan, Fangchao"

作者地址："[Liu, Yu; Yu, Haitao; Gou, Xin; Ren, Ke; Yuan, Fangchao] Chongqing Med Univ, Dept Urol, Affiliated Hosp 1, 1 Youyi Rd, Chongqing 400016, Peoples R China; [Liu, Yu] Chongqing Tradit Chinese Med Hosp, Dept Urol, Chongqing, Peoples R China; [Zeng, Bin] Chongqing Med Univ, Dept Breast & Thyroid Surg, Affiliated Hosp 2, Chongqing, Peoples R China"

通信作者："Ren, K; Yuan, FC (通讯作者)，Chongqing Med Univ, Dept Urol, Affiliated Hosp 1, 1 Youyi Rd, Chongqing 400016, Peoples R China."

来源：CANCER BIOMARKERS

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001004140800005

JCR分区：Q3

影响因子：2.2

年份：2023

卷号：37

期号：2

开始页：121

结束页：131

文献类型：Article

关键词：miR-383-5p; PI3KR1; AKT; bladder cancer

摘要："BACKGROUND: MicroRNAs have been proven to be key molecules in human malignancy. However, to our knowledge, there is no study reporting miR-383-5p expression level and the role it plays in bladder cancer (BC). METHODS: We identified miR-383-5p to be one of the tumor-suppressing genes through using data from The Cancer Genome Atlas (TCGA) and GEO database. We evaluate the expression and activity of miR-383-5p in both BC tissue and cell lines. The impacts of miR-383-5p on proliferative, migratory ability and apoptotic rate in BC cell were evaluated by utilizing CCK-8 kits, flow cytometry, and Transwell assays. qRT-PCR, western blot, and luciferase reporter assays have been adopted to investigate the underlying mechanisms. In vivo tumorigenicity testing was conducted to determine the impact of miR-383-5p on BC cellular proliferative capacity. RESULTS: Reduced miR-383-5p expression has been determined in BC tissue than in normal bladder tissue. Furthermore, BC cell proliferative, migratory ability was inhibited while apoptosis enhanced in vitro and in vivo by miR-383-5p up-regulation. In vitro and in vivo, silencing miR-383-5p considerably improved the growth and invasive capacity of cell, while decreased the apoptotic rates of BC cells. CONCLUSION: miR-383-5p plays its role as a tumor-suppressing gene by suppressing the PI3K/AKT signaling, hence preventing the development of BC."

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