Engineering bacteriophages for enhanced host range and efficacy: insights from bacteriophage-bacteria interactions

作者全名："Jia, Huang-Jie; Jia, Pan-Pan; Yin, Supei; Bu, Ling-Kang; Yang, Guan; Pei, De-Sheng"

作者地址："[Jia, Huang-Jie] Univ Chinese Acad Sci, Coll Resources & Environm, Beijing, Peoples R China; [Jia, Huang-Jie; Yang, Guan] Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing, Peoples R China; [Jia, Pan-Pan; Pei, De-Sheng] Chongqing Med Univ, Sch Publ Hlth, Chongqing, Peoples R China; [Yin, Supei] Chongqing Med Univ, Urinary Nephropathy Ctr, Affiliated Hosp 2, Chongqing, Peoples R China; [Bu, Ling-Kang] Henan Normal Univ, Coll Life Sci, Xinxiang, Peoples R China"

通信作者："Pei, DS (通讯作者)，Chongqing Med Univ, Sch Publ Hlth, Chongqing, Peoples R China."

来源：FRONTIERS IN MICROBIOLOGY

ESI学科分类：MICROBIOLOGY

WOS号：WOS:001004256400001

JCR分区：Q2

影响因子：4

年份：2023

卷号：14

期号：　

开始页：　

结束页：　

文献类型：Review

关键词：multidrug resistant bacteria; phage engineering; gene editing; phage-host interaction; high-throughput methods

摘要："Bacteriophages, the most abundant organisms on earth, have the potential to address the rise of multidrug-resistant bacteria resulting from the overuse of antibiotics. However, their high specificity and limited host range can hinder their effectiveness. Phage engineering, through the use of gene editing techniques, offers a means to enhance the host range of bacteria, improve phage efficacy, and facilitate efficient cell-free production of phage drugs. To engineer phages effectively, it is necessary to understand the interaction between phages and host bacteria. Understanding the interaction between the receptor recognition protein of bacteriophages and host receptors can serve as a valuable guide for modifying or replacing these proteins, thereby altering the receptor range of the bacteriophage. Research and development focused on the CRISPR-Cas bacterial immune system against bacteriophage nucleic acids can provide the necessary tools to promote recombination and counter-selection in engineered bacteriophage programs. Additionally, studying the transcription and assembly functions of bacteriophages in host bacteria can facilitate the engineered assembly of bacteriophage genomes in non-host environments. This review highlights a comprehensive summary of phage engineering methods, including in-host and out-of-host engineering, and the use of high-throughput methods to understand their role. The main aim of these techniques is to harness the intricate interactions between bacteriophages and hosts to inform and guide the engineering of bacteriophages, particularly in the context of studying and manipulating the host range of bacteriophages. By employing advanced high-throughput methods to identify specific bacteriophage receptor recognition genes, and subsequently introducing modifications or performing gene swapping through in-host recombination or out-of-host synthesis, it becomes possible to strategically alter the host range of bacteriophages. This capability holds immense significance for leveraging bacteriophages as a promising therapeutic approach against antibiotic-resistant bacteria."

基金机构："Chongqing Medical University Talent Project [R4014, R4020]; National Natural Science Foundation (NSFC) of China [32200386]; China-Sri Lanka Joint Research and Demonstration Center for Water Technology, China-Sri Lanka Joint Center for Education and Research, Chinese Academy of Sciences, China"

基金资助正文："Funding This work was supported by the Chongqing Medical University Talent Project (Nos. R4014 to D-SP, and R4020 to P-PJ), National Natural Science Foundation (NSFC) of China (No. 32200386 to P-PJ), and China-Sri Lanka Joint Research and Demonstration Center for Water Technology, China-Sri Lanka Joint Center for Education and Research, Chinese Academy of Sciences, China."