Laryngeal Cartilage Regeneration of Nude Rats by Transplantation of Mesenchymal Stem Cells Derived from Human-Induced Pluripotent Stem Cells

作者全名："Mizuno, Keisuke; Ohnishi, Hiroe; Yoshimatsu, Masayoshi; Zhao, Chengzhu; Hayashi, Yasuyuki; Kuwata, Fumihiko; Kaba, Shinji; Okuyama, Hideaki; Kawai, Yoshitaka; Hiwatashi, Nao; Kishimoto, Yo; Sakamoto, Tatsunori; Ikeya, Makoto; Omori, Koichi"

作者地址："[Mizuno, Keisuke; Ohnishi, Hiroe; Yoshimatsu, Masayoshi; Hayashi, Yasuyuki; Kuwata, Fumihiko; Kaba, Shinji; Okuyama, Hideaki; Kawai, Yoshitaka; Hiwatashi, Nao; Kishimoto, Yo; Sakamoto, Tatsunori; Omori, Koichi] Kyoto Univ, Grad Sch Med, Dept Otolaryngol Head & Neck Surg, 54 Shogoin Kawahara Cho,Sakyo Ku, Kyoto 6068507, Japan; [Yoshimatsu, Masayoshi] Kagoshima Univ, Grad Sch Med & Dent Sci, Dept Otolaryngol Head & Neck Surg, Kagoshima, Japan; [Zhao, Chengzhu; Ikeya, Makoto] Kyoto Univ, Ctr iPS Cell Res & Applicat, Dept Clin Applicat, Kyoto, Japan; [Zhao, Chengzhu] Chongqing Med Univ, Inst Life Sci, Lab Skeletal Dev & Regenerat, Chongqing, Peoples R China; [Okuyama, Hideaki] McGill Univ, Sch Commun Sci & Disorders, Montreal, PQ, Canada; [Sakamoto, Tatsunori] Shimane Univ, Fac Med, Dept Otorhinolaryngol, Matsue, Japan"

通信作者："Omori, K (通讯作者)，Kyoto Univ, Grad Sch Med, Dept Otolaryngol Head & Neck Surg, 54 Shogoin Kawahara Cho,Sakyo Ku, Kyoto 6068507, Japan."

来源：CELL TRANSPLANTATION

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001005252500001

JCR分区：Q2

影响因子：3.2

年份：2023

卷号：32

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：human-induced pluripotent stem cells; thyroid cartilage; regeneration; mesenchymal stem cell; nude rat

摘要："Previous studies transplanted human-induced pluripotent stem cells (hiPSCs)-derived mesenchymal stem cells (iMSCs) into thyroid cartilage defect of X-liked severe combined immunodeficiency (X-SCID) rats and confirmed transplanted cell survival and cartilage regeneration. Thus, this study aimed to investigate the contribution of iMSC transplantation to thyroid cartilage regeneration of nude rats. iMSCs were induced from hiPSCs via a neural crest cell lineage. Then, clumps formed from an iMSC/extracellular matrix complex were transplanted into thyroid cartilage defects in nude rats. The larynx was removed and histological and immunohistochemical analyses were performed 4 or 8 weeks after the transplantation. Human nuclear antigen (HNA)-positive cells were observed in 11 of 12 (91.7%) rats, which indicated that transplanted iMSCs survived in thyroid cartilage defects in nude rats. HNA-positive cells co-expressed SOX9, and type II collagen was identified around HNA-positive cells in 8 of 12 rats (66.7%), which indicated cartilage-like regeneration. Cartilage-like regeneration in nude rats in this study was comparable to the previous report on X-SCID rats (HNA-positive cells were observed in all 14 rats and cartilage-like regeneration was observed in 10 of 14 rats). This result suggests that nude rats could be an alternative to X-SCID rats in thyroid cartilage regeneration experiments using iMSCs, and this nude rat cartilage transplantation model may develop cartilage regeneration research concerning fewer problems such as infection due to immunosuppression."

基金机构："Japan Society for the Promotion of Science [19K18803, 22K16898]; Alumni otolaryngology fund from the Department of Otolaryngology Head and Neck Surgery, Graduate School of Medicine, Kyoto University"

基金资助正文："The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported by the Grant-in-Aid for Early-Career Scientists from Japan Society for the Promotion of Science [grant number 19K18803, 22K16898]; Alumni otolaryngology fund from the Department of Otolaryngology Head and Neck Surgery, Graduate School of Medicine, Kyoto University."