A smartphone-connected point-of-care photochemical biosensor for the determination of whole blood creatinine by differential optical signal readout

作者全名："Cheng, Jie; Guo, Jiuchuan; Li, Xiaosong; Guo, Jinhong"

作者地址："[Cheng, Jie; Guo, Jinhong] Shanghai Jiao Tong Univ, Sch Sensing Sci & Engn, Shanghai 200240, Peoples R China; [Cheng, Jie; Guo, Jinhong] Univ Elect Sci & Technol China, Sch Informat & Commun Engn, Chengdu 611731, Peoples R China; [Guo, Jiuchuan] Univ Elect Sci & Technol China, Sch Automat Engn, Chengdu 611731, Peoples R China; [Guo, Jinhong] Chongqing Med Univ, Coll Lab Med, MOE Key Lab Lab Med Diagnost, Chongqing 400016, Peoples R China; [Li, Xiaosong] Chongqing Med Univ, Affiliated Hosp 1, Coll Lab Med, Clin Mol Med Testing Ctr, Chongqing, Peoples R China"

通信作者："Guo, JH (通讯作者)，Shanghai Jiao Tong Univ, Sch Sensing Sci & Engn, Shanghai 200240, Peoples R China.; Li, XS (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Coll Lab Med, Clin Mol Med Testing Ctr, Chongqing, Peoples R China."

来源：BIOSENSORS & BIOELECTRONICS

ESI学科分类：CHEMISTRY

WOS号：WOS:001007015200001

JCR分区：Q1

影响因子：10.7

年份：2023

卷号：235

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：POC; Creatinine bioassay; Differential optical signal; Colorimetric; Smartphone-connected biosensor; Endogenous interference

摘要："The level of creatinine in the human body has clinical implications with regard to a potential association with kidney, muscle, and thyroid dysfunction, hence necessitating fast and accurate detection, especially at the pointof-care (POC) level. This paper presents the design, fabrication, and feasibility of a compact, low-cost and reliable POC photochemical biosensor connected to a smartphone for the determination of whole blood creatinine by differential optical signal readout. Disposable, dual-channel paper-based test strips were fabricated using stackable multilayer films pre-immobilized with enzymes and reagents for the identification and conversion of creatinine and creatine, resulting in dramatic colorimetric signals. A handheld optical reader was integrated with dual-channel differential optical readout to address endogenous interferences in the enzymatic assay of creatinine. We demonstrated this differential concept with spiked blood samples, obtaining a wide detection range of 20-1483 mu mol/L and a low detection limit of 0.03 mu mol/L. Further interference experiments displayed the differential measuring system's excellent performance against endogenous interference. Furthermore, the sensor's high reliability was confirmed through comparison with the laboratory method, with the results of 43 clinical tests consistent with the bulky automatic biochemical analyzer, with its correlation coefficient R2 = 0.9782. Additionally, the designed optical reader is Bluetooth-enabled and can connect to a cloud-based smartphone to transmit test data, enabling active health management or remote monitoring. We believe the biosensor has the potential to be an alternative to the current creatinine analysis conducted in hospitals and clinical laboratories, and it has promising prospects for contributing to the development of POC devices."

基金机构：National Natural Sci- ence Foundation of China [62122017]

基金资助正文：The authors thank the financial support from National Natural Sci- ence Foundation of China (62122017) .