A novel cysteine catalytic oxidation-based colorimetric approach for sensitive analysis of acute pancreatitis-related microRNA

作者全名："Gong, Mingwei; Jiang, Guoyan; Rong, Yuan; Yang, Zeping; She, Dong; Sun, Chunhong"

作者地址："[Gong, Mingwei; Jiang, Guoyan; Rong, Yuan; Yang, Zeping; She, Dong; Sun, Chunhong] Chongqing Med Univ, Affiliated Hosp 3, Emergency Dept, 1 Shuanghu Branch Rd, Chongqing 401120, Peoples R China"

通信作者："Sun, CH (通讯作者)，Chongqing Med Univ, Affiliated Hosp 3, Emergency Dept, 1 Shuanghu Branch Rd, Chongqing 401120, Peoples R China."

来源：JOURNAL OF ANALYTICAL SCIENCE AND TECHNOLOGY

ESI学科分类：CHEMISTRY

WOS号：WOS:001008673400001

JCR分区：Q3

影响因子：2.5

年份：2023

卷号：14

期号：1

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结束页：　

文献类型：Article

关键词：Rolling circle amplification; Gold nanoparticles (AuNPs); microRNAs (miRNAs); Cysteine

摘要："It is essential to establish simple, sensitive and accurate quantitative approaches for microRNAs (miRNAs) identification due to its crucial roles in a variety of physiological and pathological processes. Herein, we propose a novel RCA-based colorimetric method for sensitive and reliable miRNA analysis. In this approach, cyclization of the padlock sequence by miRNA-21 initiates the RCA to produce numerous G-rich sequences. The generated G-rich sequences fold to G-quadruplex DNAzyme that is capable of catalyzing cysteine to cystine which could mediate the gold nanoparticle-based color reaction, outputting results that can be observed directly by naked eyes. Based on this, the approach exhibits a wide detection range with a low limit of detection of 4 fM. In addition, the dual target recognition endows the method a greatly improved selectivity."

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