Differentially expressed miRNA profiles of serum-derived exosomes in patients with sudden sensorineural hearing loss

作者全名："Zhang, Juhong; Ma, Haizhu; Yang, Guijun; Ke, Jing; Sun, Wenfang; Yang, Li; Kuang, Shaojing; Li, Hai; Yuan, Wei"

作者地址："[Zhang, Juhong; Ma, Haizhu; Yang, Guijun; Ke, Jing; Sun, Wenfang; Yang, Li; Kuang, Shaojing; Yuan, Wei] Chongqing Gen Hosp, Dept Otorhinolaryngol Head & Neck Surg, Chongqing, Peoples R China; [Zhang, Juhong; Ke, Jing; Yuan, Wei] Chongqing Med Univ, Sch Basic Med, Chongqing, Peoples R China; [Li, Hai] Xuanhan Cty Peoples Hosp, Dept Otorhinolaryngol Head & Neck Surg, Dazhou, Sichuan, Peoples R China"

通信作者："Yuan, W (通讯作者)，Chongqing Gen Hosp, Dept Otorhinolaryngol Head & Neck Surg, Chongqing, Peoples R China.; Yuan, W (通讯作者)，Chongqing Med Univ, Sch Basic Med, Chongqing, Peoples R China."

来源：FRONTIERS IN NEUROLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001008799300001

JCR分区：Q2

影响因子：2.7

年份：2023

卷号：14

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：sudden sensorineural hearing loss; exosomes; miRNA transcriptome sequencing; miRNA; biomarkers

摘要："ObjectivesThis study aimed to compare the expressed microRNA (miRNA) profiles of serum-derived exosomes of patients with sudden sensorineural hearing loss (SSNHL) and normal hearing controls to identify exosomal miRNAs that may be associated with SSNHL or serve as biomarkers for SSNHL. MethodsPeripheral venous blood of patients with SSNHL and healthy controls was collected to isolate exosomes. Nanoparticle tracking analysis, transmission electron microscopy, and Western blotting were used to identify the isolated exosomes, after which total RNA was extracted and used for miRNA transcriptome sequencing. Differentially expressed miRNAs (DE-miRNAs) were identified based on the thresholds of P < 0.05 and |log(2)fold change| > 1 and subjected to functional analyses. Finally, four exosomal DE-miRNAs, including PC-5p-38556_39, PC-5p-29163_54, PC-5p-31742_49, and hsa-miR-93-3p_R+1, were chosen for validation using quantitative real-time polymerase chain reaction (RT-qPCR). ResultsExosomes were isolated from serum and identified based on particle size, morphological examination, and expression of exosome-marker proteins. A total of 18 exosomal DE-miRNAs, including three upregulated and 15 downregulated miRNAs, were found in SSNHL cases. Gene ontology (GO) functional annotation analysis revealed that target genes in the top 20 terms were mainly related to ""protein binding,"" ""metal ion binding,"" ""ATP binding,"" and ""intracellular signal transduction."" Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis revealed that these target genes were functionally enriched in the ""Ras,"" ""Hippo,"" ""cGMP-PKG,"" and ""AMPK signaling pathways."" The expression levels of PC-5p-38556_39 and PC-5p-29163_54 were significantly downregulated and that of miR-93-3p_R+1 was highly upregulated in SSNHL. Consequently, the consistency rate between sequencing and RT-qPCR was 75% and sequencing results were highly reliable. ConclusionThis study identified 18 exosomal DE-miRNAs, including PC-5p-38556_39, PC-5p-29163_54, and miR-93-3p, which may be closely related to SSNHL pathogenesis or serve as biomarkers for SSNHL."

基金机构：e Chongqing Science and Health Joint General Project [2022MSXM046]; Medical Science and Technology Innovation Fund of Chongqing People's Hospital (Key Project) [Y2020ZDXM01]; High-end Medical Talents of Young and Middle-aged People in Chongqing; Young and Middle-aged Medical Excellence Team in Chongqing; Health Joint Major Medical Project [2022DBXM006]

基金资助正文："This study was supported by the Chongqing Science and Health Joint General Project (No. 2022MSXM046), the Medical Science and Technology Innovation Fund of Chongqing People's Hospital (Key Project) (No. Y2020ZDXM01), High-end Medical Talents of Young and Middle-aged People in Chongqing (7th, Organization Department of Chongqing Municipal Committee of CPC, Chongqing Municipal Health Commission), the Young and Middle-aged Medical Excellence Team in Chongqing (the first batch) Chongqing Science, and the Health Joint Major Medical Project (No. 2022DBXM006)."