YES-associated protein-regulated Smad7 worsen epithelial barrier injury of chronic sinusitis with nasal polyps

作者全名："Jiang, Xiaocong; Shu, Longlan; Liu, Yijun; Shen, Yang; Ke, Xia; Liu, Jie; Yang, Yucheng"

作者地址："[Jiang, Xiaocong; Shu, Longlan; Liu, Yijun; Shen, Yang; Ke, Xia; Liu, Jie; Yang, Yucheng] Chongqing Med Univ, Affiliated Hosp 1, Dept Otorhinolaryngol, Chongqing, Peoples R China; [Yang, Yucheng] Chongqing Med Univ, Affiliated Hosp 1, Dept Otorhinolaryngol, 1 Youyi Rd, Chongqing 400016, Peoples R China"

通信作者："Yang, YC (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Otorhinolaryngol, 1 Youyi Rd, Chongqing 400016, Peoples R China."

来源：IMMUNITY INFLAMMATION AND DISEASE

ESI学科分类：IMMUNOLOGY

WOS号：WOS:001009646300001

JCR分区：Q3

影响因子：3.1

年份：2023

卷号：11

期号：6

开始页：　

结束页：　

文献类型：Article

关键词：CRSwNP; nasal epithelial barrier; Smad7; TGF-beta 1; YAP

摘要："Background: Chronic rhinosinusitis with nasal polyps (CRSwNP) was potentially due to the epithelial barrier injury. YES-associated protein (YAP) is a multifunctional transcriptional factor and plays versatile roles in the regulation and maintenance of epithelial barrier in different organs and tissues. The purpose of this study is to elucidate possible effect and mechanism of YAP on the epithelial barrier of CRSwNP. Methods: Patients were divided into CRSwNP group (n = 12) and control group (n = 9). The location of YAP, PDZ-binding transcriptional co-activator (TAZ), and Smad7 were estimated by immunohistochemistry and immunofluorescence. Meanwhile, the expression of YAP, TAZ, Zona occludens-1 (ZO-1), E-cadherin, and transforming growth factor-beta1 (TGF-beta 1) were detected by Western blot. After primary human nasal epithelial cells were treated with YAP inhibitor, the expression level of YAP, TAZ, ZO-1, E-cadherin, TGF-beta 1, and Smad7 were measured by Western blot. Results: Compared with the control group, the protein levels of YAP, TAZ, and Smad7 were significantly upregulated, while TGF-beta 1, ZO-1, and E-cadherin were downregulated in CRSwNP. YAP and Smad7 demonstrated lower levels, while the expression of ZO-1, E-cadherin, and TGF-beta 1 rose slightly after YAP inhibitor treatment in primary nasal epithelial cells. Conclusions: Higher level of YAP may lead to CRSwNP epithelial barrier injury via the TGF-beta 1 signaling pathway, and the inhibition of YAP can partially reverse epithelial barrier function."

基金机构：Chongqing Talents Project; Chongqing Middle and Youth Medical High-end Talent Studio Project [2]; National Natural Science Foundation of China [81970864]

基金资助正文："The Chongqing Talents Project, Grant/Award Number: Yu Wei (2021); Chongqing Middle and Youth Medical High-end Talent Studio Project, Grant/Award Number: Yu Wei (2018) No. 2; National Natural Science Foundation of China, Grant/Award Number: 81970864"