Chitin nanocrystal-assisted 3D bioprinting of gelatin methacrylate scaffolds

作者全名："Ling, Zhengyun; Zhao, Jian; Song, Shiyu; Xiao, Shuwei; Wang, Pengchao; An, Ziyan; Fu, Zhouyang; Shao, Jinpeng; Zhang, Zhuang; Fu, Weijun; Song, Shenghan"

作者地址："[Ling, Zhengyun; Fu, Weijun] Nankai Univ, Sch Med, Tianjin 300071, Peoples R China; [Ling, Zhengyun; Wang, Pengchao; An, Ziyan; Fu, Zhouyang; Shao, Jinpeng; Fu, Weijun] Peoples Liberat Army Gen Hosp, Med Ctr 3, Dept Urol, Beijing 100039, Peoples R China; [Zhao, Jian; Wang, Pengchao; An, Ziyan; Fu, Zhouyang; Shao, Jinpeng] Med Sch PLA, Beijing 100853, Peoples R China; [Zhao, Jian] 960th Hosp PLA, Dept Urol, Jinan 250031, Peoples R China; [Song, Shiyu] Chongqing Med Univ, Undergrad Student Majoring Clin Pharm, Chongqing 400016, Peoples R China; [Song, Shiyu] AF Med Ctr, Dept Urol, Beijing 100142, Peoples R China; [Zhang, Zhuang] Beijing Inst Basic Med Sci, Beijing 100850, Peoples R China; [Song, Shenghan] Capital Med Univ, Beijing Chaoyang Hosp, Dept Vasc Surg, Beijing 100020, Peoples R China"

通信作者："Fu, WJ (通讯作者)，Nankai Univ, Sch Med, Tianjin 300071, Peoples R China.; Fu, WJ (通讯作者)，Peoples Liberat Army Gen Hosp, Med Ctr 3, Dept Urol, Beijing 100039, Peoples R China.; Song, SH (通讯作者)，Capital Med Univ, Beijing Chaoyang Hosp, Dept Vasc Surg, Beijing 100020, Peoples R China."

来源：REGENERATIVE BIOMATERIALS

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:001013320100001

JCR分区：Q1

影响因子：5.6

年份：2023

卷号：10

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：3D bioprinting; gelatin methacrylate; chitin nanocrystal; hydrogels; scaffolds

摘要："In recent years, there has been an increasing focus on the application of hydrogels in tissue engineering. The integration of 3D bioprinting technology has expanded the potential applications of hydrogels. However, few commercially available hydrogels used for 3D biological printing exhibit both excellent biocompatibility and mechanical properties. Gelatin methacrylate (GelMA) has good biocompatibility and is widely used in 3D bioprinting. However, its low mechanical properties limit its use as a standalone bioink for 3D bioprinting. In this work, we designed a biomaterial ink composed of GelMA and chitin nanocrystal (ChiNC). We explored fundamental printing properties of composite bioinks, including rheological properties, porosity, equilibrium swelling rate, mechanical properties, biocompatibility, effects on the secretion of angiogenic factors and fidelity of 3D bioprinting. The results showed that adding 1% (w/v) ChiNC to 10% (w/v) GelMA improved the mechanical properties and printability of the GelMA hydrogels, promoted cell adhesion, proliferation and vascularization and enabled the printing of complex 3D scaffolds. This strategy of incorporating ChiNC to enhance the performance of GelMA biomaterials could potentially be applied to other biomaterials, thereby expanding the range of materials available for use. Furthermore, in combination with 3D bioprinting technology, this approach could be leveraged to bioprint scaffolds with complex structures, further broadening the potential applications in tissue engineering."

基金机构："National Natural Science Foundation of China [82270721, 81873600]"

基金资助正文：This work was financially supported by the National Natural Science Foundation of China (82270721) and the National Natural Science Foundation of China (81873600).