M2 Macrophages-Derived Exosomal miRNA-23a-3p Promotes the Progression of Oral Squamous Cell Carcinoma by Targeting PTEN

作者全名："Li, Jun; Bao, Yongjie; Peng, Sisi; Jiang, Chao; Zhu, Luying; Zou, Sihai; Xu, Jie; Li, Yong"

作者地址："[Li, Jun; Bao, Yongjie; Peng, Sisi; Jiang, Chao; Zhu, Luying; Zou, Sihai; Xu, Jie; Li, Yong] Chongqing Med Univ, Coll Stomatol, Chongqing 401147, Peoples R China; [Li, Jun; Bao, Yongjie; Peng, Sisi; Jiang, Chao; Zhu, Luying; Zou, Sihai; Xu, Jie; Li, Yong] Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing 401147, Peoples R China; [Li, Jun; Bao, Yongjie; Peng, Sisi; Jiang, Chao; Zhu, Luying; Zou, Sihai; Xu, Jie; Li, Yong] Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing 401147, Peoples R China"

通信作者："Xu, J; Li, Y (通讯作者)，Chongqing Med Univ, Coll Stomatol, Chongqing 401147, Peoples R China.; Xu, J; Li, Y (通讯作者)，Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing 401147, Peoples R China.; Xu, J; Li, Y (通讯作者)，Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing 401147, Peoples R China."

来源：CURRENT ISSUES IN MOLECULAR BIOLOGY

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:001014200800001

JCR分区：Q3

影响因子：2.8

年份：2023

卷号：45

期号：6

开始页：4936

结束页：4947

文献类型：Article

关键词：OSCC; M2 macrophages; exosome; MiR-23a-3p; PTEN

摘要："Exosomes from tumor cells and immune cells regulate the tumor microenvironment through the biomolecules or microRNAs (miRNAs) they carry. This research aims to investigate the role of miRNA in exosomes derived from tumor-associated macrophages (TAMs) in the progression of oral squamous cell carcinoma (OSCC). RT-qPCR and Western blotting assays were used to determine the expression of genes and proteins in OSCC cells. CCK-8, Scratch assay and invasion-related proteins were utilized to detect the malignant progression of tumor cells. High-throughput sequencing predicted differentially expressed miRNAs in exosomes secreted by M0 and M2 macrophages. Compared with exosomes from M0 macrophages, exosomes from M2 macrophages led to enhanced proliferation and invasion of OSCC cells and inhibited their apoptosis. High-throughput sequencing results show that miR-23a-3p is differentially expressed in exosomes from M0 and M2 macrophages. MiRNA target gene database predicts that phosphatase and tensin homolog (PTEN) are target genes of miR-23a-3p. Further studies revealed that transfection of miR-23a-3p mimics inhibited PTEN expression in vivo and in vitro and promoted the malignant progression of OSCC cells, which was reversed by miR-23a-3p inhibitors. MiR-23a-3p in exosomes derived from M2 macrophages promotes malignant progression of OSCC. PTEN is a potential intracellular target of miR-23a-3p. MiR-23a-3p, an M2 macrophage-associated exosome, is a promising target for the future treatment of OSCC."

基金机构：National Natural Science Foundation of China [81800999]; Chongqing Technology Innovation and Application Development Special Project [cstc2019jscx-msxnX0173]

基金资助正文："This research was supported by the National Natural Science Foundation of China (81800999), The Chongqing Technology Innovation and Application Development Special Project (cstc2019jscx-msxnX0173)."