Prodigiosin as an antibiofilm agent against multidrug-resistant Staphylococcus aureus

作者全名："Yan, Jing; Yin, Qi; Nie, Hao; Liang, Jinyou; Liu, Xiang-Ru; Li, Yingli; Xiao, Hong"

作者地址："[Yan, Jing; Yin, Qi; Nie, Hao; Liu, Xiang-Ru; Li, Yingli; Xiao, Hong] Chongqing Med Univ, Sch Publ Hlth, Dept Hlth Lab Technol, Chongqing, Peoples R China; [Liang, Jinyou] Shenzhen Univ, Coll Life Sci & Oceanog, Shenzhen Key Lab Marine Bioresource & Ecoenvironm, Shenzhen Engn Lab Marine Algal Biotechnol, Shenzhen, Peoples R China"

通信作者："Xiao, H (通讯作者)，Chongqing Med Univ, Sch Publ Hlth, Dept Hlth Lab Technol, Chongqing, Peoples R China."

来源：BIOFOULING

ESI学科分类：PLANT & ANIMAL SCIENCE

WOS号：WOS:001015471700001

JCR分区：Q1

影响因子：2.6

年份：2023

卷号：39

期号：4

开始页：444

结束页：458

文献类型：Article

关键词：Antibiofilm; hospital-acquired infections; multidrug resistance; prodigiosin; Staphylococcus aureus

摘要："Staphylococcus aureus is known for forming bacterial biofilms that confer increased antimicrobial resistance. Combining antibiotics with antibiofilm agents is an alternative approach, but the antibiofilm ability of prodigiosin (PG), a potential antibiotic synergist, against antimicrobial-resistant (AMR) S. aureus remains to be understood. The antibiofilm activity of PG against 29 clinical AMR S. aureus strains was evaluated using crystal violet staining, and its synergistic effects with vancomycin (VAN) was confirmed using the checkerboard test. The viability and metabolic activity of biofilms and planktonic cells were also assessed. The results revealed that PG exhibited promising inhibitory activity against biofilm formation and synergistic activity with VAN. It effectively reduced the metabolic activity of biofilms and suppressed the production of exopolysaccharides, which might be attributed to the downregulation of biofilm-related genes such as sarA, agrA, and icaA. These findings suggest that PG could be used as a preventive coating or adjuvant against biofilms in clinical settings."

基金机构：National Natural Science Foundation of China [32100139]; Science and Technology Research Program of Chongqing Municipal Education Commission [KJQN202100412]; Project of Chongqing Science and Technology Bureau

基金资助正文："This work was supported by the National Natural Science Foundation of China (No. 32100139), the Science and Technology Research Program of Chongqing Municipal Education Commission (No. KJQN202100412), and the Project of Chongqing Science and Technology Bureau (No. sl202100000725)."