The Flow Cytometric Analysis of Peripheral Blood Lymphocytes and Expression of HLA II Molecules in Lymphocyte During Acute Rejection After Renal Transplantation

作者全名："Guo, Jianzhuang; Qin, Chengwei; Li, Xiangdong; Zhuang, Xiaoxuan"

作者地址："[Guo, Jianzhuang; Li, Xiangdong] Shandong First Med Univ, Shandong Prov Qianfoshan Hosp, Dept Clin Lab, Affiliated Hosp 1, Jinan, Shandong, Peoples R China; [Qin, Chengwei] Shandong Second Prov Gen Hosp, Dept Anesthesiol, Jinan, Shandong, Peoples R China; [Zhuang, Xiaoxuan] Chongqing Med Univ, Int Med Coll, Chongqing, Peoples R China"

通信作者："Li, XD (通讯作者)，Shandong First Med Univ, Shandong Prov Qianfoshan Hosp, Dept Clin Lab, Affiliated Hosp 1, Jinan, Shandong, Peoples R China.; Qin, CW (通讯作者)，Shandong Second Prov Gen Hosp, Dept Anesthesiol, Jinan, Shandong, Peoples R China."

来源：JOURNAL OF INFLAMMATION RESEARCH

ESI学科分类：IMMUNOLOGY

WOS号：WOS:001015583300001

JCR分区：Q2

影响因子：4.2

年份：2023

卷号：16

期号：　

开始页：2607

结束页：2613

文献类型：Article

关键词：acute rejection; HLA II molecules; kidney transplantation; peripheral blood lymphocytes; flow cytometric analysis

摘要："Objective: To investigate the changes in the proportion of peripheral blood lymphocytes and the expression of HLA II molecules in lymphocytes during acute rejection after renal transplantation.Methods: Thirty-five patients who had undergone renal transplantation were selected. Eighteen patients with clinical and pathological confirmed acute rejection were selected as the test group, and twelve patients without clinical acute rejection symptoms were selected as the control group. Flow cytometry analysis was used to determine the proportion of peripheral blood lymphocytes. The mRNA and protein expression of HLA II molecules on peripheral blood lymphocytes were detected using real-time fluorescence quantification and immunoblotting, respectively.Results: The proportion of T lymphocytes, B lymphocytes, and CD4CD8 double positive T cells in the Control Group were 67.48% & PLUSMN; 5.35%, 10.82% & PLUSMN; 1.26%, and 0.88% & PLUSMN; 0.06%, respectively, and in the Test Group were 87.52% & PLUSMN; 6.28%, 3.36% & PLUSMN; 0.26%, and 0.34% & PLUSMN; 0.03%, with a significant difference respectively. The mRNA and protein expressions of HLA II molecules of peripheral blood B lymphocytes in the control group were significantly higher that these in the test group.Conclusion: The proportion of peripheral blood T lymphocytes, B lymphocytes, CD4CD8 double positive T cells, and the expression of HLA II molecules of peripheral blood lymphocytes can all indicate the occurrence of acute renal transplantation rejection, which were exceedingly useful to clinicians in judging the acute rejection of renal transplantation in the early stages."

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