GlycoEVLR: Glycosylated extracellular vesicle-like receptors for targeting and sensing viral antigen

作者全名："Cui, Feiyun; Song, Yingli; Ji, Haijie; Li, Mengnan; Zhuang, Xiwei; Zeng, Chijia; Qu, Bin; Mao, Hongju; Zhang, Jufan; Zhou, H. Susan; Zhou, Qin"

作者地址："[Cui, Feiyun; Song, Yingli; Li, Mengnan; Zhou, Qin] Harbin Med Univ, Sch Biomed Sci, Harbin 150081, Peoples R China; [Ji, Haijie] Chongqing Med Univ, Sch Lab Med, Minist Educ, Key Lab Clin Diagnost, Chongqing 400016, Peoples R China; [Zhuang, Xiwei; Zeng, Chijia] Foshan Fosun Chancheng Hosp, Dept Lab Med & Pathol, Foshan 528000, Guangdong, Peoples R China; [Qu, Bin] Saarland Univ, Ctr Integrat Physiol & Mol Med CIPMM, Sch Med, Biophys, D-66421 Homburg, Germany; [Mao, Hongju] Chinese Acad Sci, Shanghai Inst Microsyst & Informat Technol, State Key Lab Transducer Technol, Shanghai 200050, Peoples R China; [Zhang, Jufan] Univ Coll Dublin, Ctr Micro Nano Mfg Technol MNMT Dublin, Sch Mech & Mat Engn, Dublin, Ireland; [Zhou, H. Susan] Worcester Polytech Inst, Dept Chem Engn, Worcester, MA 01609 USA"

通信作者："Cui, FY; Zhou, Q (通讯作者)，Harbin Med Univ, Sch Biomed Sci, Harbin 150081, Peoples R China."

来源：CHEMICAL ENGINEERING JOURNAL

ESI学科分类：ENGINEERING

WOS号：WOS:001015775500001

JCR分区：Q1

影响因子：13.3

年份：2023

卷号：469

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Extracellular vesicle-like receptor (EVLR); Electrochemical biosensors; Glycosylation; Viral antigens detection; SARS-CoV-2; Instant screening test (IST)

摘要："Biosensors are rapid and portable detection devices with great potential for the instant screening of infectious diseases. Receptors are the critical element of biosensors. They determine the specificity, sensitivity and stability. However, current receptors are mainly limited to antibodies and aptamers. Herein, we developed a glycosylated extracellular vesicle-like receptor (GlycoEVLR) for the rapid detection of virus antigens, specifically using SARSCoV-2 as a model. The human angiotensin-converting enzyme 2 (ACE2)-overexpressed and heparinfunctionalized HEK-293T cell membrane-cloaked Fe3O4 nanoparticles (NPs) were prepared as functionalizing GlycoEVLR. They were characterized as spherical core-shell structures with a diameter of around 100 nm, which were perfectly comparable to natural extracellular vesicles. Binding affinities between GlycoEVLR and spike1 (S1) antigen were demonstrated using surface plasmon resonance (SPR). The GlycoEVLR was fixed on magnetic electrodes to construct electrochemical biosensors. Using electrochemical impedance spectroscopy (EIS) as a measurement technique, the S1 antigen was detected down to 1 pg/mL within 20 min and showed a good linearity range from 1 pg/mL to 1 ng/mL. Also, the GlycoEVLR-based electrochemical biosensors showed excellent antifouling performance and stability. Overall, our work provides a useful methodology for developing extracellular vesicle-like receptors for biosensors. Combining the inherit natural receptor proteins and antifouling lipids from the host cells with engineered glycan motifs to target and sense viral antigens will open a new avenue for biosensors."

基金机构："National Natural Science Funds of China [82102502, 82030065]"

基金资助正文：The study was supported by the National Natural Science Funds of China (82102502 to Prof. Feiyun Cui and 82030065 to Prof. Qin Zhou) .