Asymmetric Hairpins DNA Encapsulated Silver Nanoclusters for In Situ Fluorescence Imaging of Fusion Gene Isoforms in Bone Marrow

作者全名："Gou, Xiaolong; Zhang, Yangli; Zhu, Shasha; Yu, Xiaolin; Qin, Lu; Cheng, Xiaoxue; Zhang, Yuhong; Ding, Shijia; Chen, Rui; Tang, Hua; Cheng, Wei"

作者地址："[Gou, Xiaolong; Zhang, Yangli; Zhu, Shasha; Qin, Lu; Cheng, Xiaoxue; Zhang, Yuhong; Cheng, Wei] Chongqing Med Univ, Affiliated Hosp 1, Ctr Clin Mol Med Detect, Chongqing 400016, Peoples R China; [Yu, Xiaolin] Zigong Fourth Peoples Hosp, Dept Lab Med, Zigong 643000, Sichuan, Peoples R China; [Ding, Shijia; Chen, Rui] Chongqing Med Univ, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China; [Tang, Hua] Chongqing Med Univ, Key Lab Mol Biol Infect Dis, Chinese Minist Educ, Chongqing 400016, Peoples R China"

通信作者："Cheng, W (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Ctr Clin Mol Med Detect, Chongqing 400016, Peoples R China."

来源：SMALL

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:001016414100001

JCR分区：Q1

影响因子：13

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：catalyzed hairpin assembly; chronic myeloid leukemia; in situ fluorescence imaging; silver nanoclusters; simultaneous detection

摘要："Rapid and accurate imaging of the BCR/ABL fusion gene isoforms (e.g., e13a2, e14a2 and co-expression type) of chronic myeloid leukemia (CML) is of vital importance to first-line drug selection, but there is no assay that meets clinical needs (e.g., clinical kits > 18 h without isoforms information). Herein, an in situ imaging platform is developed for the rapid and accurate detection of CML fusion gene isoforms using asymmetric sequence-enhanced hairpins DNA encapsulated silver nanoclusters (ADHA) and catalyzed hairpin assembly (CHA). The specific detection of e13a2 and e14a2 fusion gene isoforms with detection limits of 19.2 am (11.558 copies & mu;L-1) and 32.56 am (19.601 copies & mu;L-1) in one-pot is achieved. The feasibility of the developed assay for real-world applications are demonstrated by one-step fluorescence imaging (40 min) of e13a2, e14a2 and co-expression type in bone marrow quantitatively (International Standard: 15.66%-168.878%) and further validated by cDNA-sequencing. This work suggests that the developed imaging platform holds great potential for rapid identification of the fusion gene isoforms and isoform related treatment monitoring."

基金机构："National Natural Science Foundation of China [81873972, 82202642]; Chongqing Science Fund for Distinguished Young Scholars [cstc2019jcyjjqX0028]; Foundation for Innovative Research Groups of Chongqing Higher Education Institutions [CXQT20013]; Chongqing Talents-Innovation Leading Talents Project [CQYC20200303107]; Chongqing Postdoctoral Innovation Talent Support Program [CQBX202218]; China Postdoctoral Science Foundation [2022M710558]; Chongqing Postdoctoral Science Foundation [2021XM2036, CSTB2022NSCQ-BHX0689]; Talent Training Foundation of Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education) of College of Laboratory Medicine [JYPY202202]; Postdoctoral Science Foundation of College of Laboratory Medicine [R10020]; Sichuan Medical Scientific Project [S20076]; Chongqing medical scientific research project [2020FYYX145]"

基金资助正文："This work was funded by the National Natural Science Foundation of China (81873972, 82202642), the Chongqing Science Fund for Distinguished Young Scholars (cstc2019jcyjjqX0028), Foundation for Innovative Research Groups of Chongqing Higher Education Institutions (CXQT20013), the Chongqing Talents-Innovation Leading Talents Project (CQYC20200303107), Chongqing Postdoctoral Innovation Talent Support Program (CQBX202218), China Postdoctoral Science Foundation (2022M710558), Chongqing Postdoctoral Science Foundation (2021XM2036, CSTB2022NSCQ-BHX0689), Talent Training Foundation of Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education) of College of Laboratory Medicine (JYPY202202) and Postdoctoral Science Foundation of College of Laboratory Medicine (R10020). Sichuan Medical Scientific Project (S20076), Chongqing medical scientific research project (2020FYYX145)."